Anti-Retinoic Acid Receptor gamma 1抗体
兔多克隆抗体to Retinoic Acid Receptor gamma 1
Rabbit
适用于: WBmore details
与反应: Mouse, Rat, Human
预测可用于: Rabbit
Synthetic peptide corresponding to Retinoic Acid Receptor gamma 1 aa 40-57 (N terminal).
Sequence:
EMLSPSFRGLGQPDLPKE
(Peptide available as ab39773)
WB: MCF7, BeWo, HaCaT, HepG2 and HEK293 cell lysate. Mouse Mammary Gland and Rat brain tissue lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab5904于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa). |
Entrez Gene: 5916 Human
Entrez Gene: 19411 Mouse
Omim: 180190 Human
SwissProt: P13631 Human
SwissProt: P18911 Mouse
Unigene: 1497 Human
Unigene: 733399 Human
Unigene: 1273 Mouse
NR1B3 antibody
Nuclear receptor subfamily 1 group B member 3 antibody
RAR gamma antibody
RAR gamma isoform 1 antibody
RAR-gamma antibody
RARC antibody
Rarg antibody
RARG_HUMAN antibody
Retinoic acid receptor gamma antibody
Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/500 dilution
Lane 1 : MCF7 Nuclear enriched extract lysate
Lane 2 : BeWo Nuclear enriched extract lysate
Lane 3 : HaCaT Nuclear enriched extract lysate
Lane 4 : HepG2 Nuclear enriched extract lysate
Lane 5 : Mouse Mammary Gland tissue lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 54 kDa
Observed band size: 60 kDawhy is the actual band size different from the predicted?
Detection: chemiluminescence.
Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
Lane 1 : HEK293 cell lysate with neutralizing peptide
Lane 2 : HEK293 cell lysate
Predicted band size: 54 kDa
Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/250 dilution + Rat brain tissue lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml
Predicted band size: 54 kDa
Observed band size: 42 kDawhy is the actual band size different from the predicted?
Detection: chemiluminescence.