Anti-Histone H3 (phospho S10)抗体
参阅全部 Histone H3 一抗
兔多克隆抗体to Histone H3 (phospho S10)
Rabbit
This antibody is specific for phosho S10 of histone H3. We believe that it does not recognise the non-modified histone - no blocking is seen with the non-phospho peptide (see Western blot image).
适用于: WB, ICCmore details
不适用于: IHC-P
与反应: Human
预测可用于: Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster, Apteronotus leptorhynchus, Cyanidioschyzon merolae, Hydractinia echinata
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab11477)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.5 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5176于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (19) | 1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
| ICC | (1) | Use at an assay dependent concentration. |
应用说明
Is unsuitable for IHC-P.
Entrez Gene: 8350 Human
Entrez Gene: 8351 Human
Entrez Gene: 8352 Human
Entrez Gene: 8353 Human
Entrez Gene: 8354 Human
Entrez Gene: 8355 Human
Entrez Gene: 8356 Human
Entrez Gene: 8357 Human
Entrez Gene: 8358 Human
Entrez Gene: 8968 Human
Entrez Gene: 319152 Mouse
Entrez Gene: 319153 Mouse
Entrez Gene: 360198 Mouse
Entrez Gene: 97908 Mouse
Omim: 602810 Human
SwissProt: P68431 Human
SwissProt: P68433 Mouse
Unigene: 132854 Human
Unigene: 247813 Human
Unigene: 247814 Human
Unigene: 248176 Human
Unigene: 443021 Human
Unigene: 484990 Human
Unigene: 532144 Human
Unigene: 533292 Human
Unigene: 546315 Human
Unigene: 586261 Human
Unigene: 591778 Human
Unigene: 221301 Mouse
Unigene: 261657 Mouse
Unigene: 377874 Mouse
Unigene: 390558 Mouse
Unigene: 397328 Mouse
Unigene: 138090 Rat
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Western blot - Anti-Histone H3 (phospho S10) antibody - ChIP Grade (ab5176)
All lanes : Anti-Histone H3 (phospho S10) antibody (ab5176) at 1/5000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa cell lysate treated with calyculin A
Lane 3 : HeLa cell lysate treated with calyculin A and alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/20000 dilution
Predicted band size: 17 kDa
Exposure time: 1 second
Immunocytochemistry - Anti-Histone H3 (phospho S10) antibody (ab5176)Yamanaka S et al. Enhanced proliferation of monolayer cultures of embryonic stem (ES) cell-derived cardiomyocytes following acute loss of retinoblastoma. PLoS ONE 3:e3896 (2008).
Images of mouse cardiomyocyte in various stages of mitosis. Puromycin-resistant cells were stained with Hoechst 33342 (blue), and probed with antibodies specific to cTnT (Green) and Histone H3 (phospho S10) (Red) using ab5176 at 1/200 dilution in ICC/IF.
Mitotic activity was confirmed by staining with an antibody to phospho-H3 Histone, which is only phosphorylated at Ser10 during mitosis. Mitotic nuclei are pink (red+blue). Bar =10 µm.
Immunocytochemistry - Anti-Histone H3 (phospho S10) antibody (ab5176)Image courtesy of Dr. Bryan Turner, United Kingdom
This image was kindly supplied by Prof Bryan Turner, University of Birmingham. Female Human Lymphoblastoid cells were incubated with ab5176.
Western blot - Anti-Histone H3 (phospho S10) antibody - ChIP Grade (ab5176)
All lanes : Anti-Histone H3 (phospho S10) antibody (ab5176) at 1 µg/ml
Lane 1 : untreated histones
Lane 2 : colcemid treated histones
Lane 3 : untreated histones with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
Lane 4 : colcemid treated histones with Human Histone H3 (phospho S10) peptide (ab11477) at 1 µg/ml
Lane 5 : untreated histones with Human Histone H3 (unmodified ) peptide (ab2903) at 1 µg/ml
Lane 6 : colcemid treated histones with Human Histone H3 (unmodified ) peptide (ab2903) at 1 µg/ml
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Exposure time: 10 seconds
Immunocytochemistry - Anti-Histone H3 (phospho S10) antibody (ab5176)This image is courtesy of an Abreview submitted by Miss Anita Ciurciu
ab5176 at a 1/100 dilution staining Drosophila melanogaster (wild type) polytene chromosomes by ICC/IF. The cells were formaldehyde fixed and blocked with 1% BSA prior to incubation with the antibody for 12 hours. Bound antibody was detected using a Cy3 conjugated goat anti-rabbit antibody.
