Anti-IRS1 (phospho S307)抗体
参阅全部 IRS1 一抗
兔多克隆抗体to IRS1 (phospho S307)
Rabbit
适用于: WB, ICCmore details
与反应: Mouse, Human
预测可用于: Rat, African green monkey
Synthetic peptide corresponding to Human IRS1 aa 300-400 (phospho S307).
WB: Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysate; NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha, whole cell lysates. ICC: T-47D cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5599于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa). | |
| ICC | Use a concentration of 1 - 2 µg/ml. |
Entrez Gene: 3667 Human
Entrez Gene: 16367 Mouse
Omim: 147545 Human
SwissProt: P35568 Human
SwissProt: P35569 Mouse
Unigene: 471508 Human
Unigene: 4952 Mouse
Unigene: 10476 Rat
HIRS 1 antibody
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Insulin receptor substrate 1 antibody
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IRS-1 antibody
IRS1 antibody
IRS1_HUMAN antibody
OTTHUMP00000164234 antibody
Immunocytochemistry - Anti-IRS1 (phospho S307) antibody (ab5599)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.25% Triton™ X-100 permeabilized T-47D cells (Human ductal breast epithelial tumor cell line) staining IRS1 with ab5599 at 1 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution (green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue) and F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control uses no primary antibody.
Western blot - Anti-IRS1 (phospho S307) antibody (ab5599)
All lanes : Anti-IRS1 (phospho S307) antibody (ab5599) at 3 µg/ml
Lane 1 : Jurkat whole cell lysates
Lane 2 : Jurkat treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysate
Lane 3 : NIH/3T3 whole cell lysates
Lane 4 : NIH/3T3 treated for 20 minutes with 50 ng/ml of TNF alpha whole cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) secondary antibody, HRP conjugate at 0.4 µg/ml
Developed using the ECL technique.
Predicted band size: 131 kDa
Observed band size: 131 kDa
Blocking buffer: 5% skimmed milk
