Anti-PYK2 (phospho Y402)抗体
参阅全部 PYK2 一抗
兔多克隆抗体to PYK2 (phospho Y402)
Rabbit
适用于: IHC-P, ICC/IF, WBmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Human PYK2 (phospho Y402). The sequence is conserved in human and rat.
IHC-P: Human brain tissue, human lung adenocarcinoma, mouse brain tissue, human hepatocarcinoma tissue. ICC/IF: Mouse mammary (NmuMG) cells, A549 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free
浓度
50 µl 浓度为 0.08 mg/ml
Immunogen affinity purified
Purified from rabbit serum by epitope specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 402.
多克隆
IgG
Abpromise™承诺保证使用ab4800于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| ICC/IF | ||
| WB |
应用说明
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK2 peptide - phospho Y402 (phospho and non-phospho pair) or CEF whole cell lysate, positive / negative for PYK 2 (pair).
ICC/IF: Use at an assay dependent dilution from (PubMed:17325207).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 2185 Human
Entrez Gene: 19229 Mouse
Omim: 601212 Human
SwissProt: Q14289 Human
SwissProt: Q9QVP9 Mouse
Unigene: 491322 Human
Unigene: 21613 Mouse
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Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)
A549 cells stained for PYK2 (green) using ab4800 at 1/500 dilution in ICC/IF. Followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)
Paraffin embedded human hepatocarcinoma tissue (right) stained for PYK2 using ab4800 at 1/50 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)
Paraffin embedded Mouse brain tissue (right) stained for PYK2 using ab4800 at 1/20 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)
Paraffin embedded human lung adenocarcinoma tissue(right) stained for PYK2 using ab4800 at 1/20 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYK2 (phospho Y402) antibody (ab4800)
Paraffin embedded human brain tissue (right) stained for PYK2 using ab4800 at 1/50 dilution in immunohistochemical analysis. Negative control (left) without primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-PYK2 (phospho Y402) antibody (ab4800)
Figure shows Pyk2 staining: Normal mouse mammary (NmuMG) cells were treated with TGFbeta and then grown to confluence, fixed and permeabilized. ab4800 was identified using a selective PSSA followed by incubation with a Cy3-conjugated anti-rabbit secondary antibody (red). F actin was visualized with Oregon Green-conjugated phalloiden (green) and paxillin staining is indicated in blue. The merged image indicates colocalization (purple) of ab4800, F-actin and paxillin in focal adhesion structures.
