Anti-Phosphotyrosine抗体[IG2]
参阅全部 Phosphotyrosine 一抗
小鼠单克隆抗体[IG2] to Phosphotyrosine
Mouse
ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides.
适用于: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.
HeLa cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Immunogen affinity purified
单克隆
IG2
IgG1
Abpromise™承诺保证使用ab5592于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA | Use a concentration of 1 µg/ml. | |
ICC/IF | 1/50 - 1/200. | |
WB | 1/100 - 1/1000. | |
ICC | 1/50 - 1/200. | |
Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
IP | Use a concentration of 2 µg/ml. |
ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)
Direct ELISA of a 96 well plate coated with 100 uL per well of phosphotyrosine, phosphoserine or phosphothreonine at 5 µg/mL, incubated with 100 µL per well of ab5592
Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)
Immunolocalization of phosphotyrosine in HeLa cells using ab5592.