Anti-Ago1抗体
参阅全部 Ago1 一抗
兔多克隆抗体to Ago1
Rabbit
适用于: IP, IHC-P, WBmore details
与反应: Mouse, Drosophila melanogaster
Synthetic peptide corresponding to Drosophila melanogaster Ago1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab23538)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5070于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | (2) | Use at an assay dependent concentration. PubMed: 18463631 |
| IHC-P | Use at an assay dependent concentration. PubMed: 18528333 | |
| WB | (3) | Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. |
Entrez Gene: 36544 Drosophila melanogaster
Entrez Gene: 236511 Mouse
SwissProt: Q8CJG1 Mouse
Unigene: 30800 Mouse
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Western blot - Anti-Ago1 antibody (ab5070)
Anti-Ago1 antibody (ab5070) at 1 µg/ml + Drosophila Melanogaster embryo nuclear extract (from melanogaster embryos 0-12Hr) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 110 kDawhy is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Western blot - Anti-Ago1 antibody (ab5070)This image is courtesy of an anonymous Abreview
Anti-Ago1 antibody (ab5070) + Drosophila Melanogaster (Fruit Fly) Tissue Lysate at 15 µg
Secondary
Sheep anti-rabbit HRP conjugated IgG Polyclonal
Performed under reducing conditions.
Predicted band size: 97 kDa
Blot was blocked with 5% Milk for 1 hour at room temperature.
