Anti-Glucocorticoid Receptor抗体
参阅全部 Glucocorticoid Receptor 一抗
兔多克隆抗体to Glucocorticoid Receptor
Rabbit
适用于: WB, ICC/IFmore details
与反应: Human
Synthetic peptide corresponding to Human Glucocorticoid Receptor aa 200-300.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3579于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | Use a concentration of 5 µg/ml. Predicted molecular weight: 97 kDa. Detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. Read More |
| ICC/IF | 1/20. |
Entrez Gene: 2908 Human
Omim: 138040 Human
SwissProt: P04150 Human
Unigene: 122926 Human
GCRST antibody
glucocorticoid nuclear receptor variant 1 antibody
Glucocorticoid receptor antibody
GR antibody
GRL antibody
Grl1 antibody
nr3c1 antibody
Nuclear receptor subfamily 3 group C member 1 antibody
nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody
GCCR antibody
GCR antibody
GCR_HUMAN antibody
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)
Immunofluorescence analysis of Glucocorticoid Receptor in MDA-MB-231 cells (serum-starved) and MDA-MB-231 cells serum-starved for 24 hours, followed by 1 µM Dexamethasone treatment for 2 hours using ab3579. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 2% BSA. The cells were labeled with ab3579 at 1/100 dilution in 0.1% BSA, incubated at 4°C overnight followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor®488 at 1/2000 dilution (Panel a: Green) in MDA-MB-231 treated cells. Nuclei (Panel b:Blue) were stained DAPI. F-actin (Panel c: Red) was stained with Rhodamine Phalloidin 1/300. (Panel d) represents the merged image showing nuclear localization of NR3C1 protein in MDA-MB-231 treated cells. (Panel e) represents the merged image of MDA-MB-231 untreated cells, that shows cytoplasmic localization of NR3C1 protein. (Panel f) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Western blot - Anti-Glucocorticoid Receptor antibody (ab3579)
All lanes : Anti-Glucocorticoid Receptor antibody (ab3579) at 1 µg/ml
Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg/ml with 5% Milk in TBST
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg with 5% Milk in TBST
Lane 5 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg with 5% Milk in TBST
Secondary
All lanes : HRP conjugate at 1/1000 dilution
Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody - ChIP Grade (ab3579)
Immunocytochemistry/Immunofluorescence analysis of A2058 (Human metastatic melanoma cell line) cells labeling Glucocorticoid Receptor (green) with ab3579 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.
Immunocytochemistry/ Immunofluorescence - Anti-Glucocorticoid Receptor antibody (ab3579)
Immunofluorescent analysis of ab3579 shows staining in HeLa (Human epithelial adenocarcinoma cell line) cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab3579at a dilution of 1/100 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
