Anti-GRP94抗体
参阅全部 GRP94 一抗
兔多克隆抗体to GRP94
Rabbit
At a very high concentration (<1/100), this antibody may recognise PDI and calreticulin. We recommend using this antibody at a relatively high dilution.
适用于: WB, IHC-P, ICC/IFmore details
与反应: Mouse, Human
Full length native protein (purified) corresponding to Dog GRP94 aa 1 to the C-terminus.
Database link: P41148
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Whole antiserum
多克隆
IgG
Abpromise™承诺保证使用ab3674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) | 1/2000 - 1/5000. Detects a band of approximately 94 kDa. Can be used at dilutions up to 1/30,000. |
| IHC-P | 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | (6) | Use at an assay dependent concentration. Used at a concentration of 1/100 for 30 min on human renal carcinoma cell (see Abreview). |
Entrez Gene: 7184 Human
Entrez Gene: 22027 Mouse
Omim: 191175 Human
SwissProt: P14625 Human
SwissProt: P08113 Mouse
Unigene: 192374 Human
Unigene: 87773 Mouse
Endoplasmin antibody
Endothelial cell (HBMEC) glycoprotein antibody
ENPL_HUMAN antibody
Glucose regulated protein 94kDa antibody
gp96 antibody
gp96 homolog antibody
GRP 94 antibody
GRP-94 antibody
Heat shock protein 90 kDa beta member 1 antibody
heat shock protein 90kDa beta (Grp94), member 1 antibody
Heat shock protein, 90 kDa, beta, 1 antibody
HSP90B1 antibody
Stress inducible tumor rejection antigen GP96 antibody
TRA1 antibody
tumor rejection antigen (gp96) 1 antibody
Tumor rejection antigen 1 antibody
Tumor rejection antigen gp96 antibody
Tumor rejection antigen-1 (gp96) antibody
94 kDa glucose regulated protein antibody
94 kDa glucose-regulated protein antibody
ECGP antibody
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)
Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).
Western blot - Anti-GRP94 antibody (ab3674)
Anti-GRP94 antibody (ab3674) at 1/2000 dilution + Cell lysates prepared from mouse fibroblasts
Western blot of mouse fibroblasts using ab3764 at 1/2000.
Immunocytochemistry/ Immunofluorescence - Anti-GRP94 antibody (ab3674)This image is courtesy of an anonymous Abreview
ab3674 at a 1/100 dilution staining human renal cell carcinoma cells by ICC/IF. The cells were permeabilized with 0.2% triton X100 and then blocked with 1%BSA, 4% goat serum prior to incubation with the primary 1antibody for 30 min at 37 °C. Bound antibody was detected using an Alexa fluor ® 488 Goat anti-Rabbit IgG (H+L).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP94 antibody (ab3674)
Ab3674 (1/1000) staining GPR94 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of peptic and parietal cells of the gastric gland.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Anti-GRP94 antibody (ab3674)Image courtesy of an anonymous Abreview.
All lanes : Anti-GRP94 antibody (ab3674) at 1/2000 dilution
Lane 1 : Whole cell lysate prepared from murine 3T3 cells
Lane 2 : Whole cell lysate prepared from murine 3T3 cells, treated with 2.5 ug/ml Tunicamycin 10 hours
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 94 kDawhy is the actual band size different from the predicted?
Exposure time: 30 seconds
