Anti-SMARCA6抗体
参阅全部 SMARCA6 一抗
兔多克隆抗体to SMARCA6
Rabbit
适用于: WBmore details
与反应: Human
Synthetic peptide (Human) - which represents a portion of human Helicase Lymphoid Specific encoded in part by exons 15 and 16.
WB: HeLa whole cell lysate
DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
DNA helicases (Hel) play a role in a number of processes involving DNA strand separation, including replication, repair, recombination, and transcription. Jarvis et al. (1996) identified a mouse gene encoding a novel putative helicase. They designated the gene LSH, for lymphoid-specific Hel, because Northern blot analysis revealed that it was expressed specifically in mouse early thymocytes. Geiman et al. (1998) determined that the mouse LSH, or HELLS (helicase lymphoid-specific), gene contains at least 18 exons and spans more than 26.6 kb. Using Northern blot analysis, these authors found that human HELLS is expressed in testis and thymus, two tissues that are highly active in DNA recombination. Geiman and Muegge (2000) demonstrated that LSH is not obligatory for normal lymphoid development but is essential for normal proliferation of peripheral T lymphocytes.
多克隆
IgG
Abpromise™承诺保证使用ab3851于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) | 1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
Entrez Gene: 3070 Human
GenBank: AAH30963 Human
Omim: 603946 Human
SwissProt: Q9NRZ9 Human
Unigene: 655830 Human
HELLS_HUMAN antibody
LSH antibody
Lymphoid specific helicase antibody
Lymphoid-specific helicase antibody
Nbla10143 antibody
PASG antibody
Proliferation associated SNF2 like protein antibody
Proliferation-associated SNF2-like protein antibody
SWI/SNF2 related matrix associated actin dependent regulator of chromatin subfamily A member 6 antibody
SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6 antibody
FLJ10339 antibody
Helicase lymphoid specific antibody
Hells antibody
Western blot - Anti-SMARCA6 antibody (ab3851)
All lanes : Anti-SMARCA6 antibody (ab3851) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 97 kDa
Exposure time: 3 minutes
Western blot - Anti-SMARCA6 antibody (ab3851)
Anti-SMARCA6 antibody (ab3851) at 1 µg/ml + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 102 kDa, 120 kDa, 47 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
