Anti-E tag抗体
参阅全部 E tag 一抗
兔多克隆抗体to E tag
Rabbit
适用于: ICC/IFmore details
与反应: Species independent
Synthetic peptide corresponding to E tag conjugated to keyhole limpet haemocyanin.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS
浓度
250 µg 浓度为 1 mg/ml
250 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3397于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. |
E epitope tag antibody
GAPVPYPDPLEPR epitope tag antibody
GAPVPYPDPLEPR tag antibody
Immunocytochemistry/ Immunofluorescence - Anti-E tag antibody (ab3397)
Immunofluorescent analysis of 4% paraformaldehyde (PFA)- fixed, permeabilized with 0.1%Triton X-100 in CHO cells transfected with 12-tags constructs (CHO-12 tags) labelling E tag with ab3397 at 1/1000 dilution, followed by Donkey Anti-rabbit IgG (H&L), FITC conjugated antibody at 1/100 dilution (Green) or Donkey Anti-rabbit IgG (H&L), Alexa Fluor594 antibody at 1/500 dilution (Red).
Nucleus was counterstained with DAPI (Blue).
Images also show parallel staining in parental CHO cell line.
Immunocytochemistry - Anti-E tag antibody (ab3397)This image is a courtesy of Anonymous Abreview
ab3397 staining E tag in cultured human HUVEC cells by Immunocytochemistry. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 1% BSA was done for 45 minutes at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA/PBS) for 16 hours at 4°C. An FITC-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.
