Anti-Ryanodine Receptor antibody [34C]

• 产品名称

  Anti-Ryanodine Receptor抗体[34C]
  参阅全部 Ryanodine Receptor 一抗

• 描述

  小鼠单克隆抗体[34C] to Ryanodine Receptor

• 宿主

  Mouse

• 特异性

  Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms. In chickens, this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibody detects the alpha isoform. By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebellar Purkinje neurons.

• 经测试应用

  适用于: IHC-P, ICC/IF, IHC-Fr, IP, Inhibition Assay, WBmore details

• 种属反应性

  与反应: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Human, Pig, Xenopus laevis, Non human primates
  预测可用于: Fish, Amphibian

• 免疫原

  Full length protein corresponding to Chicken Ryanodine Receptor. Partially purified chicken pectoral muscle ryanodine receptor.

• 阳性对照

• rat skeletal muscle

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• Primary antibody说明

  The Ryanodine Receptor (RyR) is the channel responsible for calcium release from muscle cell Sarcoplasmic Reticulum (SR) and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains. The remainder of the protein, termed the "foot" region, is located in the cytoplasm between the transverse tubule and the SR. Mammalian RyR isoforms are the product of three different genes: RyR-1 is expressed predominantly in skeletal muscle and areas of the brain; RyR-2 is expressed predominantly in heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain; and RyR-3 is found in smooth muscle and the brain (striatum, thalamus and hippocampus). In non-mammalian vertebrates, the RyR isoforms are termed alpha, beta and cardiac which correlate loosely to the mammalian RyR-1, RyR-3 and RyR-2 isoforms respectively.

• 克隆

  单克隆

• 克隆编号

  34C

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2868于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 281473 Cow

• Entrez Gene: 403615 Dog

• Entrez Gene: 606491 Dog

• Entrez Gene: 6261 Human

• Entrez Gene: 6262 Human

• Entrez Gene: 6263 Human

• Entrez Gene: 20190 Mouse

• Entrez Gene: 20191 Mouse

• Entrez Gene: 20192 Mouse

• Entrez Gene: 396718 Pig

• Entrez Gene: 396856 Pig

• Entrez Gene: 396888 Pig

• Entrez Gene: 114207 Rat

• Entrez Gene: 170546 Rat

• Entrez Gene: 689560 Rat

• Omim: 180901 Human

• Omim: 180902 Human

• Omim: 180903 Human

• SwissProt: P21817 Human

• SwissProt: Q15413 Human

• SwissProt: Q92736 Human

• SwissProt: E9PZQ0 Mouse

• SwissProt: Q14AQ9 Mouse

• SwissProt: Q80X16 Mouse

• SwissProt: Q80ZZ5 Mouse

• SwissProt: P16960 Pig

• SwissProt: Q29104 Pig

• SwissProt: Q29105 Pig

• SwissProt: P11716 Rabbit

• SwissProt: P30957 Rabbit

• SwissProt: F1LMY4 Rat

• Unigene: 466664 Human

• Unigene: 439745 Mouse

• Unigene: 225306 Rat

• Unigene: 87520 Rat

别名

• Arrhythmogenic right ventricular dysplasia 2 antibody

• ARVC2 antibody

• ARVD2 antibody

• Brain ryanodine receptor calcium release channel antibody

• Brain type ryanodine receptor antibody

• Cardiac muscle ryanodine receptor calcium release channel antibody

• Cardiac muscle type ryanodine receptor antibody

• CCO antibody

• Central core disease of muscle antibody

• HBRR antibody

• hRYR 2 antibody

• hRYR2 antibody

• MHS antibody

• MHS1 antibody

• Ryanodine receptor 1 (skeletal) antibody

• Ryanodine receptor 1 antibody

• Ryanodine receptor 2 (cardiac) antibody

• Ryanodine receptor 2 antibody

• Ryanodine receptor 3 antibody

• Ryanodine receptor type1 antibody

• RYDR antibody

• RYR 1 antibody

• RYR 2 antibody

• RYR 3 antibody

• RYR antibody

• RYR-1 antibody

• RyR1 antibody

• RYR1_HUMAN antibody

• RYR2 antibody

• RYR3 antibody

• Sarcoplasmic reticulum calcium release channel antibody

• Skeletal muscle calcium release channel antibody

• Skeletal muscle ryanodine receptor antibody

• Skeletal muscle type ryanodine receptor antibody

• Skeletal muscle-type ryanodine receptor antibody

• SKRR antibody

• Type 1 like ryanodine receptor antibody

• Type 1 ryanodine receptor antibody

• VTSIP antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)

  ICC/IF image of ab2868 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2868, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)Image courtesy of an Abreview submitted by Jenny Sharpe

  ab2868 staining Ryanodine Receptor (red) in Mouse Skeletal muscle cells at day 10 of agrin-treated differentation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 10% serum for 45 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 3% BSA) for 2 hours at 20°C. An Alexa Fluor® 647-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nucleus stained blue.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [34C] (ab2868)

  Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Ryanodine Receptor ab2868 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [34C] (ab2868)Image courtesy of an Abreview submitted by Can Zhou

  ab2868 staining Ryanodine Receptor in Rat cardiomyocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol/acetone (1:1) and blocked with 3% BSA for 1 hour at 18°C. Samples were incubated with primary antibody (1/300 in PBS + 3% BSA) for 16 hours at 4°C. An Alexa Fluor® 546-conjugated Goat anti-mouse IgG (H+L) polyclonal (1/300) was used as the secondary antibody.

  See Abreview