Anti-Rabphilin 3A抗体
参阅全部 Rabphilin 3A 一抗
兔多克隆抗体to Rabphilin 3A
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat
Synthetic peptide corresponding to Rat Rabphilin 3A aa 1-18.
Sequence:
MTDTVVNRWMYPGDGPLQ
(Peptide available as ab4954)
WB: Rat Brain ; Mouse Brain ICC/IF: RSC96
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3338于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | Use a concentration of 1 µg/ml. By Western blot, this antibody detects an ~75 kDa protein representing Rabphilin 3A from rat brain whole protein extract. |
| ICC/IF | Use a concentration of 2 µg/ml. |
Entrez Gene: 19894 Mouse
SwissProt: P47708 Mouse
Unigene: 181166 Mouse
Unigene: 10976 Rat
KIAA0985 antibody
Rabphilin 3A homolog antibody
Rabphilin-3A antibody
Rabphilin3A antibody
RP3A antibody
RP3A_HUMAN antibody
Rph 3a antibody
Rph3a antibody
Exophilin 1 antibody
Exophilin-1 antibody
Exophilin1 antibody
Western blot - Anti-Rabphilin 3A antibody (ab3338)
All lanes : Anti-Rabphilin 3A antibody (ab3338) at 3 µg/ml
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lane 3 : Rat Lung
Lane 4 : Rat Liver
Lane 5 : Rat Kidney
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/4000 dilution
Additional bands at: ~76 kDa. We are unsure as to the identity of these extra bands.
Detection: chemiluminescence
Rabphilin 3A observed in Mouse and Rat Brain, and not observed in other tissues which are documented to be Rabphilin 3A negative.
Western blot - Anti-Rabphilin 3A antibody (ab3338)
Anti-Rabphilin 3A antibody (ab3338) at 2 µg/ml + Rat Brain
Secondary
HRP conjugated Goat anti-Rabbit IgG at 1/2500 dilution
Additional bands at: ~76 kDa. We are unsure as to the identity of these extra bands.
Detection: chemiluminescence
Western blot - Anti-Rabphilin 3A antibody (ab3338)
Western blot detection of Rabphilin 3A in rat brain extract using ab3338.
Immunocytochemistry/ Immunofluorescence - Anti-Rabphilin 3A antibody (ab3338)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase RSC96 (rat neuronal Schwann cell) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling Rabphilin 3A with ab3338 at 2 µg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.
