Anti-KAT13A / SRC1抗体
参阅全部 KAT13A / SRC1 一抗
兔多克隆抗体to KAT13A / SRC1
Rabbit
Detects steroid receptor coactivator-1 (SRC-1).
适用于: ICC, ICC/IF, IHC-Frmore details
与反应: Mouse, Rat, Human
预测可用于: Pig
Synthetic peptide corresponding to Human KAT13A/ SRC1 aa 1-100.
Database link: Q15788
(Peptide available as ab5848)
ICC: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate ICC/IF: A549 (Human lung carcinoma cell line) whole cell lysate IHC-Fr: Mouse brain ; Rat Brain
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab2859于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| ICC/IF | (1) | Use a concentration of 1 µg/ml. |
| IHC-Fr | (1) | Use a concentration of 1 µg/ml. |
Entrez Gene: 8648 Human
Entrez Gene: 17977 Mouse
Omim: 602691 Human
SwissProt: Q15788 Human
SwissProt: P70365 Mouse
Unigene: 596314 Human
Unigene: 301039 Mouse
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Immunocytochemistry - Anti-KAT13A / SRC1 antibody (ab2859)
Immunolocalization of SRC1 in HeLa cells using ab2859.
Immunocytochemistry/ Immunofluorescence - Anti-KAT13A / SRC1 antibody (ab2859)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.25% Triton™ X-100 permeabilized, 70% confluent log phase A549 (Human lung carcinoma cell line) cells, blocked with 3% BSA-PBS for 1 hour at room temperature. Labelling KAT13A / SRC1 with ab2859 at 1 µg/mL and incubated for 3 hours at room temperature then labelled with secondary antibody goat anti-rabbit igG, Alexa Fluor® 488 conjugate at a 1/2000 dilution for 45 minutes at room temperature (Green). Nuclei stained with DAPI (Blue). F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)This image is courtesy of an Abreview submitted by Dr Jiqiang Zhang
ab2859 at 1/2000 staining mouse brain tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 72 hours. A biotinylated goat anti-rabbit antibody was used as the secondary.
Immunohistochemistry (Frozen sections) - Anti-KAT13A / SRC1 antibody (ab2859)
Immunohistochemitry (Frozen sections) analysis of SRC1 in the Dentate Gyrus region of the rat brain using ab2859.
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