Anti-Blood Group Lewis y antibody [F3]

• 产品名称

  Anti-Blood Group Lewis y抗体[F3]
  参阅全部 Blood Group Lewis y 一抗

• 描述

  小鼠单克隆抗体[F3] to Blood Group Lewis y

• 宿主

  Mouse

• 特异性

  This antibody belongs to the BG-8 group of murine monoclonal antibodies and is specific against the Lewis y (Type 2 Chain) antigen.

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  SK-LU-3 lung cancer cell line (Human).

• 阳性对照

• IHC-P: Human colon and lung tumor tissues.

• 常规说明

  
  

  Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituents: PBS, 1% BSA

• 纯度

  Ammonium Sulphate Precipitation

• Primary antibody说明

  Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. Lewis y (type 2 chain) antigen is a difucosylated tetrasaccharide found on the Type 2 blood group oligosaccharides of glycolipids and glycoproteins. It is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

• 克隆

  单克隆

• 克隆编号

  F3

• 同种型

  IgM

The Abpromise guarantee

Abpromise™承诺保证使用ab3359于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 别名

• Lewis Y blood group antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (ab3359)This image is courtesy of an Abreview submitted by David Ivancic

  Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of formaldehyde-fixed human colon tissue permeabilized with 0.05% Tween 20. Stained with ab3359 at 1/50 dilution. Secondary antibody used was Alexa fluor® 647 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. Blocking was done with Sea Block for 30 minutes at 22°C. The sample was incubated with the primary antibody and Sea Block for 14 hours at 4°C. Antigen retrieval method was heat mediated, ab64236 100x Citrate Buffer pH 6.0.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (ab3359)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tumor tissue labeling Blood Group Lewis y with ab3359 at 1/40 dilution for 20 minutes at room temperature.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Blood Group Lewis y antibody [F3] (ab3359)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tumor tissue labeling Blood Group Lewis y with ab3359 at 1/40 dilution for 20 minutes at room temperature.