Anti-CACNA1S antibody [1A]

• 产品名称

  Anti-CACNA1S抗体[1A]
  参阅全部 CACNA1S 一抗

• 描述

  小鼠单克隆抗体[1A] to CACNA1S

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ELISA, Inhibition Assay, ICC/IF, Flow Cyt, Flow Cyt (Intra), IP, IHC-P, IHC-Frmore details

• 种属反应性

  与反应: Mouse, Rat, Rabbit, Guinea pig, Human
  

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Human skeletal muscle tissue

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.4
  Preservative: 0.05% Sodium azide

• 浓度

• 200 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• Primary antibody说明

  Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

• 克隆

  单克隆

• 克隆编号

  1A

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2862于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 779 Human

• Entrez Gene: 12292 Mouse

• Entrez Gene: 682930 Rat

• Omim: 114208 Human

• SwissProt: Q13698 Human

• SwissProt: Q02789 Mouse

• SwissProt: Q02485 Rat

• Unigene: 1294 Human

• Unigene: 4418 Mouse

• Unigene: 10738 Rat

• Unigene: 220579 Rat

别名

• alpha-1 polypeptide antibody

• CAC1S_HUMAN antibody

• CACH1 antibody

• Cach1b antibody

• CACN1 antibody

• CACNA1S antibody

• CACNL1A3 antibody

• Calcium channel antibody

• Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal antibody

• Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle antibody

• Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle, hypokalemic periodic paralysis antibody

• Calcium channel, skeletal muscle dihydropyridine sensitive , alpha 1 subunit antibody

• Calcium channel, voltage dependent, L type, alpha 1S subunit antibody

• Calcium channel, voltage-dependent, L type, alpha 1S subunit, b antibody

• Cav1.1 antibody

• CCHL1A3 antibody

• Dihydropyridine receptor antibody

• Dihydropyridine sensitive L type calcium channel alpha 1 subunit antibody

• fmd antibody

• HOKPP antibody

• HypoPP antibody

• isoform 3 antibody

• L type antibody

• Malignant hyperthermia susceptibility 5 antibody

• mdg antibody

• MHS5 antibody

• ROB1 antibody

• sj antibody

• skeletal muscle antibody

• TTPP1 antibody

• Voltage gated calcium channel subunit alpha Cav1.1 antibody

• Voltage-dependent L-type calcium channel subunit alpha-1S antibody

• Voltage-gated calcium channel subunit alpha Cav1.1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human skeletal muscle tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then incubated with ab2862 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Flow Cytometry - Anti-CACNA1S antibody [1A] (ab2862)

  Flow cytometry analysis of Dihydropyridine Receptor alpha-1 in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5 x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Dihydropyridine Receptor alpha-1 monoclonal antibody at 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.