Anti-ERp57抗体
参阅全部 ERp57 一抗
兔多克隆抗体to ERp57
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human, Chinese hamster
Tissue, cells or virus corresponding to Rat ERp57.
Database link: P11598
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab13507于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) | 1/1000. Predicted molecular weight: 57 kDa. This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis. |
| ICC/IF | (3) | 1/1000. |
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Unigene: 591095 Human
Unigene: 263177 Mouse
Unigene: 11527 Rat
Entrez Gene: 100689343 Chinese hamster
Entrez Gene: 2923 Human
Entrez Gene: 14827 Mouse
Omim: 602046 Human
SwissProt: P30101 Human
SwissProt: P27773 Mouse
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Western blot - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyan
Anti-ERp57 antibody (ab13507) at 1/2000 dilution + Human LNCaP whole cell lysate at 25 µg
Secondary
HRP-conjugated Donkey anti-rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55 kDawhy is the actual band size different from the predicted?
Exposure time: 45 seconds
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyan
ab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)
ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-ERp57 antibody (ab13507)
All lanes : Anti-ERp57 antibody (ab13507) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from CHO-K1 cells
Lane 3 : Cell lysates prepared from human liver microsomes
Lane 4 : Cell lysates prepared from rat liver microsomes
Lane 5 : Cell lysates prepared from mouse liver microsomes
Predicted band size: 57 kDa
