Anti-PLK1抗体[AZ44]
参阅全部 PLK1 一抗
小鼠单克隆抗体[AZ44] to PLK1
Mouse
This is a useful antibody to check that extracts have maintained their CSF status and haven't been activated into interphase.
适用于: WBmore details
与反应: Human, Xenopus laevis
不与反应: Mouse
Recombinant full length protein (Xenopus laevis).
This antibody is thought to bind to a phosphorylated epitope. However, this has not yet been proved, but it does stain in a mitotic specific manner.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS
浓度
50 µg 浓度为 1 mg/ml
50 µg 浓度为 1.1 mg/ml
Protein A purified
单克隆
AZ44
Sp2/0-Ag14
IgG1
Abpromise™承诺保证使用ab12212于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) | Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 68 kDa). |
Entrez Gene: 5347 Human
Entrez Gene: 380481 Xenopus laevis
Omim: 602098 Human
SwissProt: P53350 Human
SwissProt: P70032 Xenopus laevis
Unigene: 592049 Human
Unigene: 28141 Xenopus laevis
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Western blot - Anti-PLK1 antibody [AZ44] (ab12212)This image is courtesy of Julian Gannon, CRUK
Western blot using ab12212.
Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extract
AZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.
Western blot using ab12212.
Lane 1: Mitotic CSF Xenopus egg extract
Lane 2: Interphase Xenopus egg extract
Lane 3: HeLa cell extract
AZ44 only blots the Xenopus mitotic form of Plx which we assume to be due to this antibody recognizing a phosphorylated epitope.