Anti-DIAPH2/DIA抗体
参阅全部 DIAPH2/DIA 一抗
兔多克隆抗体to DIAPH2/DIA
Rabbit
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Rabbit, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide within Human DIAPH2/DIA aa 50-150. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O60879
WB: HEK-293T, HeLa and Jurkat whole cell lysates. IHC-P: Human testis tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab12319于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (1) | 1/1000 - 1/10000. Predicted molecular weight: 132 kDa. |
Entrez Gene: 1730 Human
Entrez Gene: 100347936 Rabbit
Omim: 300108 Human
SwissProt: O60879 Human
Unigene: 226483 Human
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Western blot - Anti-DIAPH2/DIA antibody (ab12319)
All lanes : Anti-DIAPH2/DIA antibody (ab12319) at 0.1 µg/ml
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Predicted band size: 132 kDa
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (ab12319)
IHC image of ab12319 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12319, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
