Anti-Ataxin 7 antibody

• 产品名称

  Anti-Ataxin 7抗体
  参阅全部 Ataxin 7 一抗

• 描述

  兔多克隆抗体to Ataxin 7

• 宿主

  Rabbit

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse

• 免疫原

  Synthetic peptide corresponding to Human Ataxin 7 aa 1-17.
  Sequence:

  MSERAADDVRGEPRRAA

  
  (Peptide available as ab39761)

  Run BLAST with Run BLAST with 

• 阳性对照

• ICC/IF: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate ; U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate WB: Transfected COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysate

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab11434于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6314 Human

• Entrez Gene: 246103 Mouse

• Omim: 164500 Human

• Omim: 607640 Human

• SwissProt: O15265 Human

• SwissProt: Q8R4I1 Mouse

• Unigene: 476595 Human

• Unigene: 133625 Mouse

• 别名

• ATXN 7 antibody

• ATXN7 antibody

• OPCA III antibody

• OPCA3 antibody

• SCA 7 antibody

• SCA7 antibody

• Spinocerebellar Ataxia 7 antibody

• Spinocerebellar ataxia type 7 protein antibody

• ADCAII antibody

• Ataxin-7 antibody

• ATX7_HUMAN antibody

• 

  Western blot - Anti-Ataxin 7 antibody (ab11434)

  Western blot of Ataxin 7 in transfected COS-1 cell lysate using ab11434.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (ab11434)

  ICC/IF image of ab11434 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11434, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 7 antibody (ab11434)

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton™ X-100 permeabilized, 70% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells, treated with 100 ng of Nocodazole for 16 hours. Labelling Ataxin 7 with ab11434 at 2 µg/mL and incubated for 3 hours at room temperature and then labelled with Goat anti-Rabbit IgG secondary antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Green). Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (Blue). F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin at a dilution of 1/300 (Red). Panel e is untreated cell with nuclear localization. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.