Anti-Laminin抗体
参阅全部 Laminin 一抗
兔多克隆抗体to Laminin
Rabbit
In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.
适用于: Dot blot, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat, Horse, Dog, Pig, Xenopus laevis, Mammals, Amphibian
Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.027% PBS, 1% BSA
浓度
125 µl 浓度为 0.7 mg/ml
125 µl 浓度为 0.54 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab11575于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot | 1/1000. This concentration was determined using laminin at 50 ng per dot. | |
| IHC-P | (27) | Use at an assay dependent concentration. |
Entrez Gene: 10319 Human
Entrez Gene: 284217 Human
Entrez Gene: 3908 Human
Entrez Gene: 3912 Human
Entrez Gene: 3913 Human
Entrez Gene: 3915 Human
Entrez Gene: 3918 Human
Entrez Gene: 16772 Mouse
Omim: 150320 Human
SwissProt: P07942 Human
SwissProt: P24043 Human
SwissProt: P25391 Human
SwissProt: P55268 Human
SwissProt: Q9Y6N6 Human
SwissProt: P19137 Mouse
SwissProt: Q9R0B6 Mouse
Unigene: 201805 Human
Unigene: 302362 Mouse
LAMA 1 antibody
LAMA antibody
LAMA1 antibody
LAMB1 antibody
Lamc3 antibody
LAMC3_HUMAN antibody
Laminin A chain antibody
Laminin alpha 1 antibody
Laminin alpha 1 chain antibody
Laminin B1 chain antibody
Laminin subunit beta 1 antibody
Laminin subunit gamma-3 antibody
Laminin-12 subunit gamma antibody
Laminin-14 subunit gamma antibody
Laminin-15 subunit gamma antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)This image is courtesy of an anonymous Abreview
Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde-fixed human brain tissue. Stained with ab11575 at 1/10000 dilution. Secondary antibody used was ab150065 at 1/250 dilution. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% Normal Donkey Serum for 19 hours at 4°C. Antigen retrieval method was heat mediated, Tris/EDTA.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG, FITC conjugate was used as secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG, Cy3™ conjugate was used as secondary antibody.
Dot Blot - Anti-Laminin antibody (ab11575)
Immunoblotting
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
Lanes
1. antibody dilution 1/1,000
2. antibody dilution 1/2,000
3. antibody dilution 1/4,000
4. antibody dilution 1/8,000
5. antibody dilution 1/16,000
6. antibody dilution 1/32,000
7. Negative control: without primary antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)
This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.
