Anti-PKC alpha antibody [133]

• 产品名称

  Anti-PKC alpha抗体[133]
  参阅全部 PKC alpha 一抗

• 描述

  小鼠单克隆抗体[133] to PKC alpha

• 宿主

  Mouse

• 特异性

  ab11723 recognises the alpha isoform of PKC.

• 经测试应用

  适用于: WB, IHC-Pmore details
  不适用于: ICC or ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
  (Peptide available as ab55672)

• 阳性对照

• WB: HAP1, K562, C6, HeLa, NIH/3T3 and HEK-293 cell lysate. Human kidney tissue lysate. Mouse and rat brain and spleen tissue lysate. Recombinant human PKC alpha protein (Active) (ab55672). IHC-P: Human, mouse and rat retina tissue.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 25th July 2021.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 浓度

• 100 µg 浓度为 1.098 mg/ml

• 1 mg 浓度为 1 mg/ml

• 40 µg 浓度为 1.098 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  133

• 骨髓瘤

  Sp2/0

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab11723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC or ICC/IF.

数据库链接

• Entrez Gene: 5578 Human

• Entrez Gene: 18750 Mouse

• Entrez Gene: 24680 Rat

• Omim: 176960 Human

• SwissProt: P17252 Human

• SwissProt: P20444 Mouse

• SwissProt: P05696 Rat

• Unigene: 531704 Human

• Unigene: 708867 Human

• Unigene: 222178 Mouse

• Unigene: 207908 Rat

别名

• AAG6 antibody

• Aging associated gene 6 antibody

• aPKC antibody

• KPCA_HUMAN antibody

• PKC alpha antibody

• PKC-A antibody

• PKC-alpha antibody

• PKCA antibody

• PRKACA antibody

• PRKCA antibody

• Protein Kinase C alpha antibody

• Protein kinase C alpha type antibody

• 

  Western blot - Anti-PKC alpha antibody [133] (ab11723)

  This image was generated from the hybridoma version of the product.

  Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
  Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
  Lanes 3, 7 and 11: K562 cell lysate (20 µg)
  Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
  Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
  Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
  Lanes 9, 10, 11 and 12: Merged (red and green) signal

  ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

  Immunohistochemical analysis of Paraffin-embedded human retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

   

• 

  Western blot - Anti-PKC alpha antibody [133] (ab11723)

  This image was generated from the hybridoma version of the product.

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
  Lane 3: K562 cell lysate (20 µg)
  Lane 4: HEK293 cell lysate (20 µg)
  
  Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

  This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.

• 

  Western blot - Anti-PKC alpha antibody [133] (ab11723)

  All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
  
  Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
  Lane 2 : Recombinant human PKC beta 1 protein (ab60840)
  Lane 3 : Recombinant human PKC beta 2 protein (ab60841)
  Lane 4 : Recombinant human PKC eta protein (ab60849)
  Lane 5 : Recombinant human PKC epsilon protein (ab60847)
  Lane 6 : Recombinant human PKC theta/PRKCQ protein (ab56641)
  Lane 7 : Recombinant human PKC mu/PKD protein (ab60873)
  Lane 8 : Recombinant human PKC zeta protein (ab60848)
  Lane 9 : Recombinant human PKC iota protein (ab60850)
  Lane 10 : Recombinant human PKC gamma protein (ab60842)
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
  
  Predicted band size: 76 kDa
  
  
  
  

  5% NFDM/TBST

  All positive controls 10 ng

• 

  Western blot - Anti-PKC alpha antibody [133] (ab11723)

  All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
  
  Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
  Lane 2 : Recombinant human PKC delta protein (ab60844)
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
  
  Predicted band size: 76 kDa
  
  
  Exposure time: 180 seconds
  
  
  

  5% NFDM/TBST

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

• 

  Western blot - Anti-PKC alpha antibody [133] (ab11723)

  All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
  
  Lane 1 : Human kidney tissue lysate 20 ug
  Lane 2 : Mouse brain tissue lysate 20 ug
  Lane 3 : Mouse spleen tissue lysate 20 ug
  Lane 4 : Rat brain tissue lysate 20 ug
  Lane 5 : Rat spleen tissue lysate 20 ug
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
  
  Predicted band size: 76 kDa
  
  
  Exposure time: 180 seconds
  
  
  

  5% NFDM/TBST

• 

  Western blot - Anti-PKC alpha antibody [133] (ab11723)

  All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution
  
  Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate 20 µg
  Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate 20 µg
  Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg
  
  Secondary
  All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
  
  Predicted band size: 76 kDa
  
  
  
  

  Exposure time

  Lane 1: 136 seconds
  Lane 2 and 3: 15 seconds

  Fresh lysates were used in lane 2 and 3.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

  Immunohistochemical analysis of paraffin-embedded rat retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.