Anti-MDC1抗体
参阅全部 MDC1 一抗
兔多克隆抗体to MDC1
Rabbit
适用于: IHC-P, WBmore details
与反应: Human
Synthetic peptide within MDC1 aa 900-1000. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q14676
WB: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support.
多克隆
IgG
Abpromise™承诺保证使用ab11170于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 220 kDa). |
Entrez Gene: 9656 Human
GenBank: NP_055456 Human
Omim: 607593 Human
SwissProt: Q14676 Human
Unigene: 653495 Human
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Western blot - Anti-MDC1 antibody (ab11170)
Lane 1 : Anti-MDC1 antibody (ab11170) at 4 µg/ml
Lane 2 : Anti-MDC1 antibody (ab11170) at 2 µg/ml
Lane 3 : Anti-MDC1 antibody (ab11170) at 1 µg/ml
Lane 4 : Anti-MDC1 antibody (ab11170) at 0.5 µg/ml
All lanes : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 220 kDa
Exposure time: 5 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody (ab11170)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MDC1 with ab11170 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody (ab11170)
IHC image of MDC1 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11170, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
