Anti-TRF2 + TRF1 antibody [TRF-78]

• 产品名称

  Anti-TRF2 + TRF1抗体[TRF-78]

• 描述

  小鼠单克隆抗体[TRF-78] to TRF2 + TRF1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length protein corresponding to Human TRF1. Produced in baculovirus.
  Database link: P54274

• 阳性对照

• ICC/IF: HeLa cells. WB: HeLa, HEK-293, HepG2 and U-2 OS cell lysate. HeLa nuclear cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: 0.0268% PBS

• 浓度

• 批次浓度范围 50 µl 浓度为 2 - 2.1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  TRF-78

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab10579于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7013 Human

• Entrez Gene: 7014 Human

• Omim: 600951 Human

• Omim: 602027 Human

• SwissProt: P54274 Human

• SwissProt: Q15554 Human

• Unigene: 442707 Human

• Unigene: 63335 Human

• 

  Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

  All lanes : Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/ml
  
  Lane 1 : Recombinant Human TRF1 protein cell lysate at 0.1 µg
  Lane 2 : Recombinant Human TRF1 protein cell lysate at 0.5 µg
  Lane 3 : Empty
  Lane 4 : Recombinant Human TRF2 protein cell lysate at 0.1 µg
  Lane 5 : Recombinant Human TRF2 protein cell lysate at 0.5 µg
  
  Predicted band size: 50 kDa
  Observed band size: 80 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Anti-TERF antibody [TRF-78] (ab10579) staining at 4 ug/ml, shown in green. In Western blot, ab10579 was shown to bind specifically to TERF. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TRF1 and TRF2 with ab10579. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Anti-TRF2 + TRF1 antibody [TRF-78].  The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

• 

  Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)Verified customer

  All lanes : Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 1/1000 dilution
  
  Lane 1 : SAOS2 (Human osteosarcoma cell line) cell lysate
  Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat anti Mouse polyclonal IRDye 800CW at 1/1000 dilution
  
  Predicted band size: 50 kDa
  
  
  Exposure time: 5 minutes
  
  
  

  Blocking step

  Milk as blocking agent for 2 hours · Concentration: 5% · Temperature: 21°C.

  Incubation time

  12 hours · Temperature: 4°C · Diluent: 5% milk in TBST.

  See Abreview

• 

  Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

  All lanes : Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/ml
  
  Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
  Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
  Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
  
  Secondary
  All lanes : Goat Anti-Mouse IgG-Peroxidase
  
  Predicted band size: 50 kDa
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

  Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody.  A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation.  The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.

  IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain. 
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)This image is courtesy of an anonymous Abreview

  ab10579 at 1/500 staining human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.

  See Abreview