Anti-CD11c antibody [3.9]

• 产品名称

  Anti-CD11c抗体[3.9]
  参阅全部 CD11c 一抗

• 描述

  小鼠单克隆抗体[3.9] to CD11c

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Flow Cyt: Human leucocytes. Human whole blood.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  3.9

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab11029于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3687 Human

• Omim: 151510 Human

• SwissProt: P20702 Human

• Unigene: 248472 Human

• 别名

• CD11 antigen like family member C antibody

• CD11 antigen-like family member C antibody

• CD11c antibody

• CD11c antigen antibody

• Complement component 3 receptor 4 subunit antibody

• CR4 antibody

• Integrin alpha X antibody

• Integrin alpha X chain antibody

• Integrin alpha-X antibody

• Integrin aX antibody

• Integrin subunit alpha X antibody

• integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody

• integrin, alpha X (complement component 3 receptor 4 subunit antibody

• ITAX_HUMAN antibody

• ITGAX antibody

• LEU M5 alpha subunit antibody

• Leu M5 antibody

• Leukocyte adhesion glycoprotein p150 95 alpha chain antibody

• Leukocyte adhesion glycoprotein p150 antibody

• Leukocyte adhesion receptor p150 95 antibody

• Leukocyte adhesion receptor p150 antibody

• Leukocyte surface antigen p150 95 alpha subunit antibody

• Leukocyte surface antigen p150 alpha subunit antibody

• Myeloid membrane antigen alpha subunit antibody

• p150 95 integrin alpha chain antibody

• p150 antibody

• p150/95 antibody

• SLEB6 antibody

• 95 alpha chain antibody

• 95 antibody

• CD 11c antibody

• 

  Flow Cytometry - Anti-CD11c antibody [3.9] (ab11029)

  Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab264107 (right) or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 μg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab264107 or Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (1x 106 in 100 μl at 0.2 μg/ml (1/5000)) for 30min on ice. The cells were simultaneously stained with CD14.

  The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

  Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS only (ab264107).

• 

  Flow Cytometry - Anti-CD11c antibody [3.9] (ab11029)

  Human whole blood stained with ab11029 (right) or mouse IgG1κ (left). Red blood cells of 200µl human whole blood were lysed, then cells were incubated for 30 min on ice in 1x PBS containing 10µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab11029) or mouse IgG1κ isotype (ab170190) (100µl at 10 µg/ml) for 30 min on ice.

  The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C.

  Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.

   

• 

  Flow Cytometry - Anti-CD11c antibody [3.9] (ab11029)

  Human peripheral blood lymphocytes stained with ab11029. Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lysed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were stained with anti-CD11c ab11029 (right panel) at 1/100 dilution for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (left panel) was mouse monoclonal IgG1 (ab170190) used under the same conditions.

  Acquisition of >30,000 total events were collected using a 50mW Argon Blue laser (488nm) and 530/30 bandpass filter. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable cells.