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Human VEGF Receptor 2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human VEGF Receptor 2 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CD309, FLK1, VEGFR2, KDR, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, FLK-1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 70 pg/mL
34.3 - 25000 pg/mL
Microplate
Abcam's VEGF Receptor 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF Receptor 2 in serum, plasma and cell culture supernatants. (Genecards: KDR)
This assay employs an antibody specific for Human VEGF Receptor 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF Receptor 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human VEGF Receptor 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF Receptor 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
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-20°C
Representative standard curve using ab100665
Representative standard curve using ab100665
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