| 存储条件 | -20℃ |
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Human IL-5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-5 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 5 pg/mL
2.74 - 2000 pg/mL
Microplate
Abcam's IL-5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-5 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-5 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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Representative standard curve using ab100571
Representative standard curve using ab100571
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