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Human IL-8 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-8 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 1 pg/mL
0.8 - 600 pg/mL
Microplate
Abcam's IL-8 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-8 in serum, plasma, and cell culture supernates.
This assay employs an antibody specific for Human IL-8 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL- 8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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Representative standard curve using ab100575
Representative standard curve using ab100575
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