Human MCP1 ELISA Kit

Human MCP1 ELISA Kit ab100586 is a sandwich ELISA to measure Human MCP1 in serum, plasma, cell culture supernatant with a sensitivity of 2 pg/ml.

How the assay works
This assay employs an antibody specific for Human MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human MCP1 ELISA Kit (CCL2) (ab179886) from our SimpleStep ELISA® range.

Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human MCP1 ELISA Kit ab100586 protocol summary
1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit
Human MCP1 ELISA Kit (CCL2) (ab179886)

Optimization may be required with urine samples.