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Human RAGE ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human RAGE in Human in Biofluids, Cell Culture Media samples.
隐藏别名
RAGE, AGER, Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 3 pg/mL
2.06 - 1500 pg/mL
Microplate
Abcam's RAGE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human RAGE ELISA Kit (ab190807) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
Blue Ice
-20°C
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-20°C
Representative Standard Curve using ab100632.
Representative Standard Curve using ab100632.
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