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Human I-309 ELISA Kit (CCL1) is a Sandwich (quantitative) ELISA for the measurement of Human I-309 (CCL1) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
Colorimetric
Plasma, Cell culture supernatant
Human
Sandwich (quantitative)
< 4 pg/mL
4.12 - 3000 pg/mL
Microplate
Abcam's I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human I-309 in normal serum with ab100536, only in serum spiked with Human I-309.
Optimization may be required with urine samples
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-20°C
-20°C
-20°C
Representative standard curve using ab100536
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