| 存储条件 |
|---|
| Product Name | UCP2 [B9-F1] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BMIQ4 antibody Mitochondrial uncoupling protein 2 antibody SLC25A8 antibody Solute carrier family 25 member 8 antibody UCP 2 antibody ucp2 antibody UCP2_HUMAN antibody UCPH antibody Uncoupling protein 2 antibody Uncoupling protein 2 mitochondrial proton carrier antibody |
| Product description | UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | BMIQ4 antibody Mitochondrial uncoupling protein 2 antibody SLC25A8 antibody Solute carrier family 25 member 8 antibody UCP 2 antibody ucp2 antibody UCP2_HUMAN antibody UCPH antibody Uncoupling protein 2 antibody Uncoupling protein 2 mitochondrial proton carrier antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Mitochondrion inner membrane. |
| SwissProt ID | P55851 |
|---|
Application
Fig1: Western blot analysis of UCP2 on human UCP2 recombinant protein using anti-UCP2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of UCP2 on HEK293 (1) and UCP2-hIgGFc transfected HEK293 (2) cell lysate using anti-UCP2 antibody at 1/1,000 dilution.
Application
Fig3: ICC staining UCP2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with UCP2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human UCP2 recombinant protein, UCP2-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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