After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4). ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.
Fig1: Western blot analysis of CHRNA2 on human CHRNA2 recombinant protein using anti-CHRNA2 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of CHRNA2 on HEK293 (1) and CHRNA2-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA2 antibody at 1/1,000 dilution.
Application
Fig3: Western blot analysis of CHRNA2 on different cell lysate using anti-CHRNA2 antibody at 1/1,000 dilution. Positive control： Line1: SK-N-SH Line2: SH-SY5Y Line3: Membrane protein lysate of C6 Line4: SW480
Positive Control
Human CHRNA2 recombinant protein, CHRNA2-hIgGFc transfected HEK293 cell lysate, SK-N-SH, SH-SY5Y, SW480 cell lysates, membrane protein lysate of C6.
Application Notes
WB:1:500-1:2,000
Additional Information
Form
Liquid
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
