| 存储条件 |
|---|
| Product Name | Asparagine synthetase [SC67-07] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | asnS antibody ASNS_HUMAN antibody ASNSD antibody Asparagine synthetase [glutamine-hydrolyzing] antibody Asparagine synthetase antibody Cell cycle control protein TS11 antibody Glutamine dependent asparagine synthetase 3 antibody Glutamine dependent asparagine synthetase antibody Glutamine hydrolyzing antibody Glutamine-dependent asparagine synthetase antibody OTTHUMP00000024510 antibody OTTHUMP00000204938 antibody OTTHUMP00000204939 antibody OTTHUMP00000204940 antibody OTTHUMP00000204941 antibody OTTHUMP00000204942 antibody TS11 antibody TS11 cell cycle control protein antibody |
| Product description | Glutamine-hydrolyzing asparagine synthetase is also commonly designated cell cycle control protein TS11. Asparagine synthetase plays an important role in the amino-acid biosynthesis pathway and is also important for L-asparagine biosynthesis. Via the L-glutamine route, it is involved in the synthesis of L-asparaine from L-aspartate. The protein contains one asparagine synthetase domain and one type-2 glutamine amidotransferase domain. The cell-cycle regulated gene encoding for asparagine synthetase, ts11, is necessary for G1 progression. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Alternative Names | asnS antibody ASNS_HUMAN antibody ASNSD antibody Asparagine synthetase [glutamine-hydrolyzing] antibody Asparagine synthetase antibody Cell cycle control protein TS11 antibody Glutamine dependent asparagine synthetase 3 antibody Glutamine dependent asparagine synthetase antibody Glutamine hydrolyzing antibody Glutamine-dependent asparagine synthetase antibody OTTHUMP00000024510 antibody OTTHUMP00000204938 antibody OTTHUMP00000204939 antibody OTTHUMP00000204940 antibody OTTHUMP00000204941 antibody OTTHUMP00000204942 antibody TS11 antibody TS11 cell cycle control protein antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Asparagine synthetase on different lysates using anti-Asparagine synthetase antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Human skeletal muscle
Application
Fig2: ICC staining Asparagine synthetase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Asparagine synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Asparagine synthetase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Asparagine synthetase antibody. Counter stained with hematoxylin.| Positive Control | K562, SW480, Hela, SH-SY-5Y, mouse stomach tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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