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| Product Name | TCF7L2 [SC06-90] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HMG box transcription factor 4 antibody hTCF 4 antibody hTCF-4 antibody T cell factor 4 antibody T cell specific HMG box antibody T cell specific transcription factor 4 antibody T-cell factor 4 antibody T-cell-specific transcription factor 4 antibody TCF 4 antibody TCF-4 antibody TCF4 antibody TCF7L2 antibody TCF7L2 protein antibody TF7L2_HUMAN antibody Transcription factor 7 like 2 antibody Transcription factor 7 like 2 T cell specific HMG box antibody Transcription factor 7-like 2 antibody |
| Product description | T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to β-catenin in a cooperative manner. This TCF-4/β-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes. c-Jun also interacts with TCF-4 and β-catenin, and the phosphorylation-dependent interaction between c-Jun and TCF4 regulates intestinal tumorigenesis by integrating JNK and APC/β-catenin. TCF-4 is also implicated in bipolar affective disorder. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HMG box transcription factor 4 antibody hTCF 4 antibody hTCF-4 antibody T cell factor 4 antibody T cell specific HMG box antibody T cell specific transcription factor 4 antibody T-cell factor 4 antibody T-cell-specific transcription factor 4 antibody TCF 4 antibody TCF-4 antibody TCF4 antibody TCF7L2 antibody TCF7L2 protein antibody TF7L2_HUMAN antibody Transcription factor 7 like 2 antibody Transcription factor 7 like 2 T cell specific HMG box antibody Transcription factor 7-like 2 antibody |
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| Molecular Weight(MW) | 68 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of TCF7L2 on Jurkat cells lysates using anti-TCF7L2 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining TCF7L2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TCF7L2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining TCF7L2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Jurkat, SW480, Hela, D3, human breast carcinoma tissue, human kidney tissue. |
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| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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