| 存储条件 |
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| Product Name | SGK1 [SC05-71] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | OTTHUMP00000017247 antibody Serine/threonine protein kinase SGK antibody Serine/threonine protein kinase Sgk1 antibody Serine/threonine-protein kinase Sgk1 antibody Serum and glucocorticoid regulated kinase antibody Serum/glucocorticoid regulated kinase 1 antibody Serum/glucocorticoid regulated kinase antibody Serum/glucocorticoid-regulated kinase 1 antibody SGK 1 antibody SGK antibody SGK1 antibody Sgk1 variant i3 antibody SGK1_HUMAN antibody |
| Product description | Serum- and glucocorticoid-regulated kinase (SGK), also known as SGK1, is a serine/threonine protein kinase and a member of the "AGC" subfamily, which includes protein kinases A, G, and C. SGK plays an important role in activating certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK contains a catalytic domain, which is most similar to Akt1 (also known as protein kinase B or PKB). SGK is a downstream target of PI 3-kinase-stimulated growth factor signaling, with 3-phosphoinositide-dependent protein kinase 1 (PDK1) capable of phosphorylating the activation-loop of SGK at Threonine-256. The adrenal corticosteroid hormone, Aldosterone, induces the transcription of SGK, which mediates Na + transport by stimulating epithelial sodium channel activity. The SGK promoter contains a glucocorticoid response element and an SP-1 regulatory element, and is a transcriptional target for p53. SGK is also a component of the p38 MAPK-mediated response to hyperosmotic stress. The human SGK gene maps to chromosome 6q23 and encodes the 431-amino acid SGK protein. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIPFC |
| WB:1:1,000 IHC:1:50-1:200: FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | OTTHUMP00000017247 antibody Serine/threonine protein kinase SGK antibody Serine/threonine protein kinase Sgk1 antibody Serine/threonine-protein kinase Sgk1 antibody Serum and glucocorticoid regulated kinase antibody Serum/glucocorticoid regulated kinase 1 antibody Serum/glucocorticoid regulated kinase antibody Serum/glucocorticoid-regulated kinase 1 antibody SGK 1 antibody SGK antibody SGK1 antibody Sgk1 variant i3 antibody SGK1_HUMAN antibody |
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| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Cytoplasm, Nucleus, Cell membrane, Mitochondrion. |
Application
Fig1: Western blot analysis of SGK1 on human kidney lysates using anti-SGK1 antibody at 1/1,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-SGK1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of 293 cells with SGK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Human liver tissue, human kidney tissue, mouse brain tissue, mouse liver tissue, mouse kidney tissue. |
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| Application Notes | WB:1:1,000 IHC:1:50-1:200: FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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