| 存储条件 |
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| Product Name | RAB27A [9D-7C] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GS2 antibody GTP-binding protein Ram antibody HsT18676 antibody MGC117246 antibody Mutant Ras related protein Rab-27A antibody Rab-27 antibody RAB-27A antibody RAB27 antibody RAB27A antibody RAB27A member RAS oncogene family antibody RAM antibody Ras-related protein Rab-27A antibody Ras-related protein Rab27A antibody RB27A_HUMAN antibody |
| Product description | The Rab family of low molecular weight GTPases are critical regulators of vesicular transport. Rab proteins cycle between an active GTP-bound state, which recruits specific effector proteins, and an inactive GDP-bound state. Two members of this family, Rab27a and Rab27b, have overlapping functions, but differ in tissue specificity. Rab27a is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Rab27b is also expressed in pituitary tissue, but is more significantly expressed in brain and spleen. Rab27a regulates diverse processes involving lysosome-related organelles, including melanosome motility in melanocytes and lytic granule release in cytotoxic T lymphocytes. Mutations in the Rab27a gene result in Griscelli syndrome (GS) or the corresponding mouse model ashen, a rare autosomal recessive disorder characterized by hypopigmentation, prolonged bleeding times, and platelet storage pool deficiency (2,4). In GS, Rab27a is not available to mediate the recruitment of melanosomes via the actin motor, myosin Va. The human Rab27b gene maps to chromosome 18q21.1, and encodes a protein that is involved in pituitary hormone secretion. Rab27b may be functionally redundant to Rab27a, as it can rescue Rab27a mutants. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | WBIHC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | GS2 antibody GTP-binding protein Ram antibody HsT18676 antibody MGC117246 antibody Mutant Ras related protein Rab-27A antibody Rab-27 antibody RAB-27A antibody RAB27 antibody RAB27A antibody RAB27A member RAS oncogene family antibody RAM antibody Ras-related protein Rab-27A antibody Ras-related protein Rab27A antibody RB27A_HUMAN antibody |
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| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Membrane |
| SwissProt ID | P51159 |
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Application
Fig1: Western blot analysis of RAB27A on human RAB27A recombinant protein using anti-RAB27A antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of RAB27A on HEK293 (1) and RAB27A-hIgGFc transfected HEK293 (2) cell lysate using anti-RAB27A antibody at 1/1,000 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-RAB27A antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-RAB27A antibody. Counter stained with hematoxylin.| Positive Control | Human RAB27A recombinant protein, RAB27A-hIgGFc transfected HEK293 cell lysate, human prostate cancer tissue, human liver cancer tissue. |
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| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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