| 存储条件 |
|---|
| Product Name | Puromycin Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Chemical compounds corresponding to puromycin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:12000 IHC:1:100-1:500 ICC:1:50-1:200 FC:1:500-1:1000 | |
| Species Reactivity | A wide range of other species |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
WB
Western blot analysis of lysates from RAW264.7 cells, using Puromycin Rabbit mAb at 1:8000 dilution. Raw264. 7 cells were treated by puromycin (20ug/ml) at 37℃ for 4 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using Puromycin Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HeLa cells (treated with puromycin) and HeLa cells (untreated) using puromycin Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
FC
Flow cytometry:1X10^6 Raw264.7 cells (negative control,left) and Raw264.7 cells(treated with puromycin,right) were intracellularly-stained with puromycin Rabbit mAb(2ug/mL,orange line) or ABflo® 488 Rabbit IgG isotype control (2ug/mL,blue line). Non-fluorescently stained cells was used as blank control (red line).| Application Notes | WB:1:2000-1:12000 IHC:1:100-1:500 ICC:1:50-1:200 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
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