| 存储条件 |
|---|
| Product Name | Myeloperoxidase Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Myeloperoxidase aa 550-745. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:5000-1:10000 ICC:1:100 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MPO |
|---|---|
| Gene Full Name | myeloperoxidase |
| Gene Summary | Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014] |
| Alternative Names | 84 kDa myeloperoxidase antibody 89 kDa myeloperoxidase antibody EC 1.11.1.7 antibody EC1.11.2.2 antibody fj80f04 antibody MPO antibody mpx antibody myeloid-specific peroxidase antibody Myeloperoxidase antibody Myeloperoxidase heavy chain antibody 84 kDa myeloperoxidase antibody 89 kDa myeloperoxidase antibody EC 1.11.1.7 antibody EC1.11.2.2 antibody fj80f04 antibody MPO antibody mpx antibody myeloid-specific peroxidase antibody Myeloperoxidase antibody Myeloperoxidase heavy chain antibody Myeloperoxidase light chain antibody PERM_HUMAN antibody wu:fj80f04 antibody |
| Molecular Weight(MW) | 84kDa(Observed band size: 84/55/37kDa) |
| Cellular Localization | Lysosome. |
WB
Western blot analysis of Myeloperoxidase on HL-60 cell lysates with Mouse anti-Myeloperoxidase antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-Myeloperoxidase antibody at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HL-60 cells labeling Myeloperoxidase with Mouse anti-Myeloperoxidase antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myeloperoxidase antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of Myeloperoxidase was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000-1:5000 IHC:1:5000-1:10000 ICC:1:100 FC:1:50-1:100 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
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