重组人TRAIL蛋白
参阅全部 TRAIL 蛋白酶
Assay1:Determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Assay2:Measured by its ability to induce apoptosis in LN-18 cells (human glioblastoma cells). The expected ED50 for this effect is 0.8 - 2.0 ng/ml.
> 98 % SDS-PAGE.
Sterile filtered Greater than 98% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).
Escherichia coli
Full length protein
No
Recombinant
Human
MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG
20 kDa
114 to 281
Our Abpromise guarantee covers the use of ab9960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
Functional Studies
SDS-PAGE
Lyophilized
The ED50 was determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶
Centrifuge the vial prior to opening. Reconstitute in 100ul water to a concentration of 0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Apo 2 ligand
APO 2L
Apo-2 ligand
Apo-2L
APO2L
CD253
CD253 antigen
Chemokine tumor necrosis factor ligand superfamily member 10
Protein TRAIL
TL2
TNF Related Apoptosis Inducing Ligand
TNF related apoptosis inducing ligand TRAIL
TNF-related apoptosis-inducing ligand
TNF10_HUMAN
TNFSF10
TRAIL
Tumor necrosis factor (ligand) family member 10
Tumor Necrosis Factor (ligand) Superfamily Member 10
Tumor necrosis factor apoptosis inducing ligand splice variant delta
Tumor necrosis factor ligand superfamily member 10