Anti-CCL21抗体
参阅全部 CCL21 一抗
兔多克隆抗体to CCL21
Rabbit
适用于: IHC-P, Neutralising, Sandwich ELISA, WB, ELISAmore details
与反应: Human
Recombinant full length protein corresponding to Human CCL21. Highly pure (>98%) recombinant human Exodus-2
Database link: O00585
Recombinant human CCL21 protein (ab9852) can be used as a positive control in WB.
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
No preservative, sterile filtered
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9851于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (2) | Use at an assay dependent concentration. |
Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of human Exodus-2 (100 ng/ml), a concentration of 6.0 - 8.0 µg/ml of this antibody is required. | |
Sandwich ELISA | Use at an assay dependent concentration. To detect CCL21-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant CCL21. Read More | |
WB | Use at an assay dependent concentration. Predicted molecular weight: 15 kDa. To detect human Exodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Exodus-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More | |
ELISA | Use at an assay dependent concentration. To detect human Exodus-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant human Exodus-2. Read More |
Entrez Gene: 6366 Human
Omim: 602737 Human
SwissProt: O00585 Human
Unigene: 57907 Human
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