Anti-Interferon gamma抗体[GIF-3]
参阅全部 Interferon gamma 一抗
小鼠单克隆抗体[GIF-3] to Interferon gamma
Mouse
适用于: Sandwich ELISA, Indirect ELISA, Inhibition Assay, WBmore details
与反应: Human
Recombinant full length protein corresponding to Interferon gamma.
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS
No preservative, sterile filtered
Protein A purified
单克隆
GIF-3
NS0/1
IgG1
unknown
Abpromise™承诺保证使用ab9801于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [GIF-10] to Interferon gamma (ab9805). PubMed: 9458493 Used as the detection antibody in conjuction with recommended pair. sELISA can be used to determine IFN-g concentration in the cultures of human peripheral blood mononuclear cells. Read More | |
Indirect ELISA | Use at an assay dependent concentration. | |
Inhibition Assay | Use at an assay dependent concentration. Can be used to neutralize the antiviral activity of IFN-g: The bioassay was performed using the L41 cell line and mouse encephalomyocarditis virus (EMCV). Briefly, interferon was pre-incubated with the ab for 2 h at RT and then added to the cell monolayer. 24 h later cells were infected with EMCV and cultured for further 24 h. Antiviral activity of interferon was evaluated using crystal-violet staining. PAbs to respective interferons and MAb isotype control were used as controls. Read More | |
WB | Use at an assay dependent concentration. |
Entrez Gene: 3458 Human
Omim: 147570 Human
SwissProt: P01579 Human
Unigene: 856 Human
ifn g antibody
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