Anti-SOCS1抗体
参阅全部 SOCS1 一抗
山羊多克隆抗体to SOCS1
Goat
适用于: Flow Cyt (Intra), ICC/IF, ICC, Flow Cyt, IHC-Pmore details
与反应: Mouse, Rat, Human, Pig
预测可用于: Cow, Dog
Synthetic peptide corresponding to Human SOCS1 aa 150-250 (C terminal).
Database link: NP_003736.1
Flow Cyt (Intra): HEK293 cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab9870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) | Use a concentration of 10 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 21628332 | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 23298838 ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody. | |
| IHC-P | Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Unigene: 130 Mouse
Unigene: 82754 Rat
Entrez Gene: 8651 Human
Entrez Gene: 12703 Mouse
Omim: 603597 Human
SwissProt: O15524 Human
SwissProt: O35716 Mouse
Unigene: 50640 Human
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Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (ab9870)
Flow cytometric analysis of HEK293 cells with ab9870 (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Human Kidney with ab9870 (8 µg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Mouse Kidney with ab9870 (8 µg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunocytochemistry - Anti-SOCS1 antibody (ab9870)
Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation with ab9870 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
