Anti-IL-12抗体
参阅全部 IL-12 一抗
山羊多克隆抗体to IL-12
Goat
适用于: ELISA, IHC-P, Sandwich ELISA, Neutralising, WBmore details
与反应: Human
Recombinant fragment corresponding to Human IL-12. p35: P29459 p40: P29460
WB: Recombinant human IL-12 p40 protein (ab9993).
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary
Shipped at 4°C. Store at +4°C.
pH: 7.40
Immunogen affinity purified
多克隆
unknown
unknown
Abpromise™承诺保证使用ab9992于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA | Use at an assay dependent concentration. To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. Read More | |
IHC-P | Use at an assay dependent concentration. | |
Sandwich ELISA | Use at an assay dependent concentration. To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. Read More | |
Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. | |
WB | (1) | Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Read More |
Entrez Gene: 3592 Human
Entrez Gene: 3593 Human
Omim: 161560 Human
Omim: 161561 Human
SwissProt: P29459 Human
SwissProt: P29460 Human
Unigene: 673 Human
CLMF1 antibody
CLMF2 antibody
Cytotoxic lymphocyte maturation factor 1, p35 antibody
Cytotoxic lymphocyte maturation factor 2 antibody
Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody
Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
IL 12A antibody
IL 2 subunit p40 antibody
IL-12 subunit p35 antibody
IL-12A antibody
IL-12B antibody
IL12 antibody
IL12, p35 subunit antibody
IL12, subunit p40 antibody
IL12A antibody
IL12A_HUMAN antibody
IL12B antibody
IL35 subunit antibody
Interleukin 12 alpha chain antibody
Interleukin 12 beta chain antibody
Interleukin 12 subunit beta antibody
Interleukin 12, p40 antibody
Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody
Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) antibody
Interleukin 12B antibody
Interleukin-12 subunit alpha antibody
Natural killer cell stimulatory factor 1, 35 kD subunit antibody
natural killer cell stimulatory factor 2 antibody
Natural killer cell stimulatory factor, 40 kD subunit antibody
NF cell stimulatory factor chain 1 antibody
NFSK antibody
NK cell stimulatory factor chain 1 antibody
NK cell stimulatory factor chain 2 antibody
NKSF antibody
NKSF1 antibody
NKSF2 antibody
P35 antibody
CLMF antibody
CLMF p35 antibody
CLMF p40 antibody