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Anti-IL-12 antibody

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产品详情
  • 产品名称

    Anti-IL-12抗体
    参阅全部 IL-12 一抗

  • 描述

    山羊多克隆抗体to IL-12

  • 宿主

    Goat

  • 经测试应用

    适用于: ELISAIHC-PSandwich ELISANeutralisingWBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to Human IL-12. p35: P29459 p40: P29460

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • WB: Recombinant human IL-12 p40 protein (ab9993).

  • 常规说明

    Although some customers have been successful in IHC we no longer batch test in this application.


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.40

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    unknown

  • 轻链类型

    unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9992于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
ELISA

Use at an assay dependent concentration. 

To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12.

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IHC-P

Use at an assay dependent concentration.

Sandwich ELISA

Use at an assay dependent concentration. 

To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12.

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Neutralising

Use at an assay dependent concentration. 

To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required.

WB(1)

Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. 

To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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  • 数据库链接

  • 别名

    • CLMF1 antibody

    • CLMF2 antibody

    • Cytotoxic lymphocyte maturation factor 1, p35 antibody

    • Cytotoxic lymphocyte maturation factor 2 antibody

    • Cytotoxic lymphocyte maturation factor 35 kDa subunit antibody

    • Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody

    • IL 12A antibody

    • IL 2 subunit p40 antibody

    • IL-12 subunit p35 antibody

    • IL-12A antibody

    • IL-12B antibody

    • IL12 antibody

    • IL12, p35 subunit antibody

    • IL12, subunit p40 antibody

    • IL12A antibody

    • IL12A_HUMAN antibody

    • IL12B antibody

    • IL35 subunit antibody

    • Interleukin 12 alpha chain antibody

    • Interleukin 12 beta chain antibody

    • Interleukin 12 subunit beta antibody

    • Interleukin 12, p40 antibody

    • Interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) antibody

    • Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) antibody

    • Interleukin 12B antibody

    • Interleukin-12 subunit alpha antibody

    • Natural killer cell stimulatory factor 1, 35 kD subunit antibody

    • natural killer cell stimulatory factor 2 antibody

    • Natural killer cell stimulatory factor, 40 kD subunit antibody

    • NF cell stimulatory factor chain 1 antibody

    • NFSK antibody

    • NK cell stimulatory factor chain 1 antibody

    • NK cell stimulatory factor chain 2 antibody

    • NKSF antibody

    • NKSF1 antibody

    • NKSF2 antibody

    • P35 antibody

    • CLMF antibody

    • CLMF p35 antibody

    • CLMF p40 antibody