Recombinant human NGF protein (Active)

• 产品名称

  重组人NGF蛋白(Active)
  参阅全部 NGF 蛋白酶

• 生物活性

  Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.

  The expected ED₅₀ is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

• 纯度

  >= 98 % SDS-PAGE.
  >=98% HPLC analyses. Sterile filtered.
  

• 内毒素水平

  < 1.000 Eu/µg

• 表达系统

  Escherichia coli

• Accession

  P01138

• 蛋白长度

  Full length protein

• 无动物成分

  No

• 性质

  Recombinant

• 
  

• 种属

  Human

• 序列

  SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA

• 预测分子量

  14 kDa

• 氨基酸

  122 to 241

• 额外的序列信息

  Full length mature protein, without the signal peptide or the propeptide. The functional form is a non-covalently linked homodimer 27 kDa.

Our Abpromise guarantee covers the use of ab9796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  HPLC

  Functional Studies

  SDS-PAGE

• 形式

  Lyophilized

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• 复溶

  Please reconstitute this product to 0.1-1 mg/ml in water. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) for long term storage.

别名

• Beta nerve growth factor

• Beta NGF

• Beta-nerve growth factor

• Beta-NGF

• HSAN5

• MGC161426

• MGC161428

• Nerve growth factor

• Nerve growth factor (beta polypeptide)

• Nerve growth factor beta

• Nerve growth factor beta polypeptide

• Nerve growth factor beta subunit

• NGF

• NGF_HUMAN

• NGFB

• NID67

• 

  Western blot - Recombinant human NGF protein (ab9796)

  All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution
  
  Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
  Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Observed band size: 140 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  
  
  

  Blocking and dilution buffer: 5% NFDM/TBST.