Anti-Histone H4抗体- ChIP Grade
参阅全部 Histone H4 一抗
兔多克隆抗体to Histone H4 - ChIP Grade
Rabbit
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
适用于: IP, ChIP, IHC-P, WBmore details
与反应: Mouse, Rat, Human
预测可用于: Chicken, Cow, Pig, Saccharomyces cerevisiae, Xenopus laevis, Caenorhabditis elegans, Drosophila melanogaster
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab13843)
For recombinant monoclonal Histone H4 antibody (ChIP grade) - please see ab213291 (IGX4696H).
For mouse monoclonal Histone H4 antibody (ChIP grade) - please see ab31830 (mAbcam 31830).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
浓度
批次浓度范围 100 µg 浓度为 0.1 - 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab10158于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| ChIP | (2) | Use 2 µg for 25 µg of chromatin. |
| IHC-P | (1) | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
| WB | (11) | Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).Can be blocked with Human Histone H4 peptide (ab13843). |
Entrez Gene: 318846 Drosophila melanogaster
Entrez Gene: 3771854 Drosophila melanogaster
Entrez Gene: 3771893 Drosophila melanogaster
Entrez Gene: 3771908 Drosophila melanogaster
Entrez Gene: 3771935 Drosophila melanogaster
Entrez Gene: 3771938 Drosophila melanogaster
Entrez Gene: 3771941 Drosophila melanogaster
Entrez Gene: 3771947 Drosophila melanogaster
Entrez Gene: 3772096 Drosophila melanogaster
Entrez Gene: 3772113 Drosophila melanogaster
Entrez Gene: 3772129 Drosophila melanogaster
Entrez Gene: 3772172 Drosophila melanogaster
Entrez Gene: 3772211 Drosophila melanogaster
Entrez Gene: 3772254 Drosophila melanogaster
Entrez Gene: 3772314 Drosophila melanogaster
Entrez Gene: 3772317 Drosophila melanogaster
Entrez Gene: 3772319 Drosophila melanogaster
Entrez Gene: 3772325 Drosophila melanogaster
Entrez Gene: 3772509 Drosophila melanogaster
Entrez Gene: 3772519 Drosophila melanogaster
Entrez Gene: 3772666 Drosophila melanogaster
Entrez Gene: 3772708 Drosophila melanogaster
Entrez Gene: 41773 Drosophila melanogaster
Entrez Gene: 121504 Human
Entrez Gene: 554313 Human
Entrez Gene: 8294 Human
Entrez Gene: 8359 Human
Entrez Gene: 8360 Human
Entrez Gene: 8361 Human
Entrez Gene: 8362 Human
Entrez Gene: 8363 Human
Entrez Gene: 8364 Human
Entrez Gene: 8365 Human
Entrez Gene: 8366 Human
Entrez Gene: 8367 Human
Entrez Gene: 8368 Human
Entrez Gene: 8370 Human
Entrez Gene: 100041230 Mouse
Entrez Gene: 319155 Mouse
Entrez Gene: 319156 Mouse
Entrez Gene: 319157 Mouse
Entrez Gene: 319158 Mouse
Entrez Gene: 319159 Mouse
Entrez Gene: 319160 Mouse
Entrez Gene: 319161 Mouse
Entrez Gene: 320332 Mouse
Entrez Gene: 326619 Mouse
Entrez Gene: 326620 Mouse
Entrez Gene: 674678 Mouse
Entrez Gene: 69386 Mouse
Entrez Gene: 97122 Mouse
Entrez Gene: 447787 Xenopus laevis
Omim: 142750 Human
Omim: 602822 Human
Omim: 602823 Human
Omim: 602824 Human
Omim: 602825 Human
Omim: 602826 Human
Omim: 602827 Human
Omim: 602828 Human
Omim: 602829 Human
Omim: 602830 Human
Omim: 602831 Human
Omim: 602833 Human
SwissProt: P84040 Drosophila melanogaster
SwissProt: P62805 Human
SwissProt: P62806 Mouse
SwissProt: P02309 Saccharomyces cerevisiae
SwissProt: P62799 Xenopus laevis
Unigene: 21500 Drosophila melanogaster
Unigene: 29514 Drosophila melanogaster
Unigene: 29527 Drosophila melanogaster
Unigene: 30219 Drosophila melanogaster
Unigene: 30220 Drosophila melanogaster
Unigene: 30221 Drosophila melanogaster
Unigene: 30223 Drosophila melanogaster
Unigene: 30868 Drosophila melanogaster
Unigene: 30869 Drosophila melanogaster
Unigene: 30871 Drosophila melanogaster
Unigene: 30872 Drosophila melanogaster
Unigene: 30873 Drosophila melanogaster
Unigene: 30876 Drosophila melanogaster
Unigene: 33873 Drosophila melanogaster
Unigene: 5747 Drosophila melanogaster
Unigene: 143080 Human
Unigene: 247816 Human
Unigene: 248172 Human
Unigene: 248178 Human
Unigene: 248179 Human
Unigene: 278483 Human
Unigene: 46423 Human
Unigene: 528055 Human
Unigene: 533295 Human
Unigene: 55468 Human
Unigene: 591790 Human
Unigene: 655235 Human
Unigene: 662174 Human
Unigene: 706635 Human
Unigene: 718935 Human
Unigene: 144300 Mouse
Unigene: 14775 Mouse
Unigene: 158272 Mouse
Unigene: 227295 Mouse
Unigene: 228709 Mouse
Unigene: 246720 Mouse
Unigene: 255646 Mouse
Unigene: 260530 Mouse
Unigene: 261642 Mouse
Unigene: 261662 Mouse
Unigene: 261664 Mouse
Unigene: 377875 Mouse
Unigene: 442307 Mouse
Unigene: 85592 Xenopus laevis
dJ160A22.1 antibody
dJ160A22.2 antibody
dJ221C16.1 antibody
dJ221C16.9 antibody
FO108 antibody
H4 antibody
H4.k antibody
H4/a antibody
H4/b antibody
H4/c antibody
H4/d antibody
H4/e antibody
H4/g antibody
H4/h antibody
H4/I antibody
H4/j antibody
H4/k antibody
H4/m antibody
H4/n antibody
H4/p antibody
H4_HUMAN antibody
H4F2 antibody
H4F2iii antibody
H4F2iv antibody
H4FA antibody
H4FB antibody
H4FC antibody
H4FD antibody
H4FE antibody
H4FG antibody
H4FH antibody
H4FI antibody
H4FJ antibody
H4FK antibody
H4FM antibody
H4FN antibody
H4M antibody
HIST1H4A antibody
HIST1H4B antibody
HIST1H4C antibody
HIST1H4D antibody
HIST1H4E antibody
HIST1H4F antibody
HIST1H4H antibody
HIST1H4I antibody
HIST1H4J antibody
HIST1H4K antibody
HIST1H4L antibody
HIST2H4 antibody
HIST2H4A antibody
Hist4h4 antibody
Histone 1 H4a antibody
Histone 1 H4b antibody
Histone 1 H4c antibody
Histone 1 H4d antibody
Histone 1 H4e antibody
Histone 1 H4f antibody
Histone 1 H4h antibody
Histone 1 H4i antibody
Histone 1 H4j antibody
Histone 1 H4k antibody
Histone 1 H4l antibody
Histone 2 H4a antibody
histone 4 H4 antibody
Histone H4 antibody
MGC24116 antibody
ChIP - Anti-Histone H4 antibody - ChIP Grade (ab10158)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab10158 (blue), and 20µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158)
All lanes : Anti-Histone H4 antibody - ChIP Grade (ab10158) at 1 µg/ml
Lane 1 : Histone prep
Lane 2 : HeLa histone lysate
Lane 3 : Histone prep with Human Histone H4 peptide (ab13843) at 1 µg/ml
Lane 4 : HeLa Histone lysate with Human Histone H4 peptide (ab13843) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 antibody - ChIP Grade (ab10158)
IHC image of Histone H4 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10158, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation - Anti-Histone H4 antibody - ChIP Grade (ab10158)
Histone H4 was immunoprecipitated using 0.5mg NIH/3T3 whole cell lysate, 5µg of Rabbit polyclonal to Histone H4 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10158.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 14kDa: Histone H4; non specific - 52 and 85kDa: We are unsure as to the identity of this extra band.
Western blot - Anti-Histone H4 antibody - ChIP Grade (ab10158)
All lanes : Anti-Histone H4 antibody - ChIP Grade (ab10158) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 14 kDawhy is the actual band size different from the predicted?
