Anti-Vimentin antibody [RV203]

• 产品名称

  Anti-Vimentin抗体[RV203]
  参阅全部 Vimentin 一抗

• 描述

  小鼠单克隆抗体[RV203] to Vimentin

• 宿主

  Mouse

• 特异性

  This antibody reacts exclusively with vimentin which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

• 经测试应用

  适用于: IHC-P, Flow Cyt, IHC-Frmore details

• 种属反应性

  与反应: Human
  不与反应: Mouse

• 免疫原

  Recombinant full length protein corresponding to Bovine Vimentin. Cytoskeletal vimentin extract of calf lens.
  Database link: P08670

• 阳性对照

• Mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma

• 常规说明

  Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  RV203

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8979于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7431 Human

• Omim: 193060 Human

• SwissProt: P08670 Human

• Unigene: 455493 Human

• Unigene: 691131 Human

• 形式

  Vimentin is found in connective tissue and in the cytoskeleton.

• 别名

• HEL113 antibody

• VIM antibody

• VIME_HUMAN antibody

• Vimentin antibody

• CTRCT30 antibody

• Epididymis luminal protein 113 antibody

• FLJ36605 antibody

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)

  Immunohistochemistry with ab8979 on a frozen section of human kidney showing positive staining in blood vessels, glomeruli and connective tissue cells and no reactivity in epithelial cells.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)

  IHC image of ab8979 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8979, µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunohistochemistry (Frozen sections) - Anti-Vimentin antibody [RV203] (ab8979)

  Immunohistochemistry on frozen section of human colon showing positive staining in connective tissue cells and no reactivity in epithelial cells

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [RV203] (ab8979)

  Immunohistochemistry on paraffin section of human colon

• 

  Flow Cytometry - Anti-Vimentin antibody [RV203] (ab8979)

  Overlay histogram showing HeLa cells stained with ab8979 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8979, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.