Anti-pan-AKT (phospho T308)抗体
参阅全部 pan-AKT 一抗
兔多克隆抗体to pan-AKT (phospho T308)
Rabbit
This antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue. Weak cross reactivity with AKT2.
适用于: IHC-P, WB, Dot blot, ELISAmore details
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
IHC-P: Human lung, testis and breast tissue. WB: Human spleen, small intestine, placenta, skeletal muscle, lung, tonsil and thymus tissue lysate. GST-tagged AKT1 recombinant protein. AKT1 and AKT3 recombinant protein.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 0.97 mg/ml
Immunogen affinity purified
AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab8933于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 56 kDa. | |
| Dot blot | Use a concentration of 5 µg/ml. | |
| ELISA | 1/15000. |
Entrez Gene: 207 Human
Entrez Gene: 11651 Mouse
Omim: 164730 Human
SwissProt: P31749 Human
SwissProt: P31750 Mouse
Unigene: 525622 Human
Unigene: 6645 Mouse
Unigene: 11422 Rat
AKT antibody
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PRKBA antibody
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PRKBG antibody
Protein kinase B antibody
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Protein kinase B gamma antibody
Proto-oncogene c-Akt antibody
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RAC antibody
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RAC PK beta antibody
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STK2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab8933)
Panel A: ab8933 (1/200 dilution, 30mins at RT) staining AKT (phospho T308) in human testis tissue in immunohistochemical analysis. Secondary used was an anti-Rabbit polyclonal HRP conjugate (Ready to use) DAB staining. Heat induced antigen retrieval was performed using Leica Bond Epitope Retrieval Buffer 1 (Citrate solution, pH6.0) for 20 minutes (ER1(20)). Counterstain is hemotoxylin.
Panel B: Secondary antibody only.
Western blot - Anti-pan-AKT (phospho T308) antibody (ab8933)
All lanes : Anti-pan-AKT (phospho T308) antibody (ab8933) at 1/2270 dilution
Lane 1 : MW Protein ladder
Lane 2 : Recombinant AKT1 protein, 50ng
Lane 3 : Recombinant AKT1 protein, 50ng (phosphatase treated)
Lane 4 : Recombinant AKT1 T308A/S473A mutant protein, 50ng
Lane 5 : Recombinant AKT2 protein, 50ng
Lane 6 : Recombinant AKT2 protein, 50ng (phosphatase treated)
Lane 7 : Recombinant AKT3 protein, 50ng
Lane 8 : Recombinant AKT3 protein, 50ng (phosphatase treated)
Predicted band size: 56 kDa
Blot A: ab8933 used at 1/2270.
Blot B: Anti-Akt used 1/1000.
Dot Blot - Anti-pan-AKT (phospho T308) antibody (ab8933)
Dot Blot - Anti-pan-AKT (phospho T308) antibody (ab8933, 5 µg/ml).
Secondary antibody is an anti-rabbit IgG HRP used at a 1/70,000 dilution.
Exposure time 60 secs.
Columns 1 – 5, Left to Right 100, 33.33, 11.11, 3.70, 1.23 ng
Row A: AKT1-BSA peptide
Row B: AKT1 pT308 – BSA peptide
Row C: AKT1 S473 – BSA peptide
Row D: AKT1 pS473 – BSA peptide
Row E: CDC27 T244 -BSA peptide
Row F: CDC27 pT244 – BSA peptide
Row G: BSA control
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab8933)
ab8933 (4µg/ml) staining AKT (phospho T308) in human breast using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane and cytoplasmic compartment within the breast ductal regions.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab8933)
ab8933 (1/200 dilution, 30mins at RT) staining AKT (phospho T308) in human lung tissue in immunohistochemical analysis. Secondary used was an anti-Rabbit polyclonal HRP conjugate (Ready to use, 8mins at RT) DAB staining. HIER using citrate buffer for 20mins. Counterstain is hemotoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT (phospho T308) antibody (ab8933)
ab8933 (1/200 dilution, 30mins at RT) staining AKT (phospho T308) in human lymph node in breast tissue in immunohistochemical analysis. Secondary used was an anti-Rabbit polyclonal HRP conjugate (Ready to use, 8mins at RT) DAB staining. HIER using citrate buffer for 20mins. Counterstain is hemotoxylin.
Western blot - Anti-pan-AKT (phospho T308) antibody (ab8933)
All lanes : Anti-pan-AKT (phospho T308) antibody (ab8933) at 1/1000 dilution (overnight at 4degC)
Lane 1 : GST-tagged AKT1 recombinant protein, 50ng
Lane 2 : GST-tagged AKT1 active recombinant protein, 50ng
Secondary
All lanes : DyLight™ 649 rabbit secondary antibody, (30mins at RT) at 1/20000 dilution
Predicted band size: 56 kDa
Recombinant protein expected to run ~80-100 kDa.
Western blot - Anti-pan-AKT (phospho T308) antibody (ab8933)
All lanes : Anti-pan-AKT (phospho T308) antibody (ab8933) at 1/1000 dilution
Lane 1 : Human spleen whole tissue lysate
Lane 2 : Human small intestine whole tissue lysate
Lane 3 : Human placenta whole tissue lysate
Lane 4 : Human skeletal muscle whole tissue lysate
Lane 5 : Human brain cerebellum whole tissue lysate
Lane 6 : Human lung whole tissue lysate
Lane 7 : Human tonsil whole tissue lysate
Lane 8 : Human thymus whole tissue lysate
Secondary
All lanes : Goat anti-Rabbit Ig HRP at 1/40000 dilution
Predicted band size: 56 kDa
Exposure time: 8 seconds
