Mouse monoclonal [7C2] Anti-Chicken IgY H&L

• 产品名称

  小鼠单克隆抗体[7C2]抗鸡IgY H&L
  参阅全部 IgY 二抗

• 宿主

  Mouse

• 靶标种属

  Chicken

• 经测试应用

  适用于: IP, WBmore details

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 0.1% BSA

• 浓度

• 250 µl 浓度为 0.1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  7C2

• 同种型

  IgG1

• 轻链类型

  unknown

• 常规说明

  The yolk of eggs laid by immunized chickens has been recognized as an excellent source of antibodies Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. Because a single egg contains as much antibody as an average bleed from a rabbit, this simple, non-invasive approach presents an appealing alternative to conventional antibody production methods. Purification of chicken egg yolk immunoglobulin Y (IgY), the 150 kDa IgG homolog, does not require animal bleeding. In addition, the eggs from immunized chickens provide a continual, daily source of antibody, and this convenient approach offers greater compatibility with animal protection regulations. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens. Highly conserved mammalian proteins sometimes fail to illicit a humoral response in animals, such as rabbits, that are traditionally used for generating antibodies. Non-specific binding and need for cross-species immunoabsorptions is eliminated since chicken IgY does not cross-react with mammalian IgG and does not bind bacterial or mammalian Fc receptors. There are well defined structural differences of IgY-type immunoglobulins and the IgG of mammals. That includes the molar mass of the heavy chains of the immunoglobulins. The IgY-type immunoglobulins are much less flexible than IgG. Also, the structures of the Fc part of the immunoglobulin isotypes IgY and IgG are different.

   

  The antibody can be used during various purification steps of IgY.

The Abpromise guarantee

Abpromise™承诺保证使用ab8922于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。