Anti-AKT1 (phospho S473)抗体
参阅全部 AKT1 一抗
兔多克隆抗体to AKT1 (phospho S473)
Rabbit
The region of AKT1 surrounding S473 has a high degree of similarity to the corresponding regions in AKT2 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue.
适用于: ICC/IF, IHC-P, WBmore details
与反应: Human
Synthetic peptide corresponding to Human AKT1 aa 450-550 (C terminal) (phospho S473) conjugated to keyhole limpet haemocyanin.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1 mg/ml
100 µg 浓度为 1.15 mg/ml
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using a phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against a non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
多克隆
IgG
Abpromise™承诺保证使用ab8932于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | 1/100 - 1/500. | |
| WB | 1/200 - 1/1000. Predicted molecular weight: 56 kDa. |
Entrez Gene: 207 Human
Omim: 164730 Human
SwissProt: P31749 Human
Unigene: 525622 Human
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Western blot - Anti-AKT1 (phospho S473) antibody (ab8932)
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.
ab8932 at a 1:200 dilution in Western Blot staining nuclear extract from cells infected with adenovirus expressing nuclear-targeted Akt kinase.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)
Normal human breast tissue. Akt is weakly phosphorylated in normal tissue in the breast. The phosphorylated Akt is clearly localised in the cytoplasm.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)
Akt is phosphorylated on Ser 473 in human breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
This image is a magnification of the accompanying breast tumor image.
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody (ab8932)
Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 (phospho S473) antibody (ab8932)
Confocal image of ab8932 at a 1:40 dilution staining cardiomyocytes infected with wild-type Akt in conjunction with a texas-red conjugated phalloidin to label filamentous actin in the cardiomyocytes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)Image from Meyer S et al., PLoS One. 2012;7(6):e38222. Epub 2012 Jun 7. Fig S5.; doi:10.1371/journal.pone.0038222; June 7, 2012, PLoS ONE 7(6): e38222.
Immunohistochemical analysis of Human prostate cancer and melanoma cell lines, staining AKT1 (phospho S473) with ab8932.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 (phospho S473) antibody (ab8932)
Akt is phosphorylated on Ser 473 in human breast tumor. The staining is much stronger than the weak basal level of phosphorylation in normal breast. The staining is cytoplasmic, as in normal tissue.
The phopho Ser 473 Akt antibody (ab8932) is used with no pretreatment at a dilution of 1:100 using Dako techmate streptavidin-biotin reagents.
