Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker

• 产品名称

  Anti-Cytokeratin 19抗体[A53-B/A2] - Cytoskeleton Marker
  参阅全部 Cytokeratin 19 一抗

• 描述

  小鼠单克隆抗体[A53-B/A2] to Cytokeratin 19 - Cytoskeleton Marker

• 宿主

  Mouse

• 特异性

  Rod domain of cytokeratin peptide 19 (40 kDa) in human tissue.

• 经测试应用

  适用于: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Cytokeratin 19 aa 1 to the C-terminus. Human mammary carcinoma cell line MCF-7
  Database link: P08727

• 表位

  Rod domain of cytokeratin peptide 19.

• 阳性对照

• ICC/IF KO: MCF7 cells (MCF7-KRT19 KO used as a negative cell line). HepG2 cells. IHC-P: Human skin. Human liver tissue. WB: HT-29 lysate. Flow Cyt (Intra): MCF7 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Do Not Freeze.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  A53-B/A2

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab7754于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3880 Human

• Omim: 148020 Human

• SwissProt: P08727 Human

• Unigene: 654568 Human

• 别名

• CK19 antibody

• Cytokeratin 19 antibody

• Cytokeratin-19 antibody

• K19 antibody

• K1C19_HUMAN antibody

• K1CS antibody

• Keratin 19 antibody

• Keratin type I 40 kD antibody

• Keratin type I 40kD antibody

• Keratin type I cytoskeletal 19 antibody

• Keratin, type I cytoskeletal 19 antibody

• Keratin, type I, 40 kd antibody

• Keratin-19 antibody

• KRT19 antibody

• MGC15366 antibody

• 40 kDa keratin intermediate filament antibody

• CK 19 antibody

• CK-19 antibody

• 

  Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using ab7754 (concentration in sample 3 μg/ml, GAM APC).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  ab7754 staining Cytokeratin 19 in wild-type MCF7 cells (top panel) and KRT19 knockout MCF7 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7754 at 1/500 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  ab7754 staining Cytokeratin 19 in human skin.
  Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  Immunohistochemical analysis of paraffin-embedded human liver tissue stained for Cytokeratin 19 using ab7754 at a 1/100 dilution followed by a GAM IgG-Alexa Fluor®488 diluted at 1/200 (green). Cell nuclei stained with PI (1 µg/ml; orange).

• 

  Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  Western blotting analysis of human cytokeratin 19 using ab7754 at 2 µg/ml on lysates of HT-29 (Human colorectal adenocarcinoma cell line) cell line and Jurkat (Human T cell leukemia cell line from peripheral blood) cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions.

  IRDye800-conjugated anti-mouse IgG1 secondary antibody.

  A specific band was detected for cytokeratin 19 at approximately 40 kDa.

• 

  Flow Cytometry (Intracellular) - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  Overlay histogram showing MCF7 cells stained with ab7754 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7754, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)

  ICC/IF image of ab7754 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7754 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)This image is courtesy of an anonymous Abreview

  ab7754 staining Cytokeratin 19 in Human liver tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% milk for 30 minutes at 37°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/1000 in antibody diluent) for 1 hour at 37°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 19 antibody [A53-B/A2] - Cytoskeleton Marker (ab7754)Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 3.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.

  Immunofluorescence analysis of 1 month hepato-differentiated Human dental pulp stem cells, staining Cytokeratin 19 with ab7754 at 1/60 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.